Near Space
D. radiodurans
Experiment
Results
100

What is the altitude range considered near space?

20-100 km

100

What is D. radiodurans famous for?

Being the most radiation-resistant organism known

100

How many flights were conducted and in which years?

Three flights — 2019, 2020 and 2021

100

Which growth medium gave the highest survival rate overall?

TGY, the richest nutrient medium

200

What are the two main causes of death for microorganisms in near space?

UV radiation and extremely low temperature

200

At what growth phase were bacteria collected for the experiments and why?

Exponential phase, because it better reflects the characteristics of the growth environment

200

What was different about the third flight compared to the previous ones?

Two devices were used in the same balloon to compare 2h and 4h exposure under the same atmospheric conditions

200

What is the relation between growth rate, manganese and paraquat with the survival rate?

Higher growth rate → higher survival

Paraquat and manganese increase survival

300

How is de panspermia hypothesis?

Life arrived from space carried by meteorites, comets, or asteroids.

300

Why is D. radiodurans in stationary phase naturally more resistant to radiation?

Because it enters a dormant state, activating general stress resistance mechanisms

300

Why was the survival of the 4h group much lower than the 2h group?

Because the 4h exposure included midday peak solar radiation, receiving far more than double the UV dose

300

By how much did survival increase in the glucose group when paraquat was added in the second flight? (%)

267%

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