Enzymes and Inhibitors
The variable that an enzyme can lower
What is activation energy?
The amount of different Amino Acids in our body.
What is 20 amino acids?
The equation for the specificity constant
What is Kcat/Km?
The purpose of amino acid sequencing.
What is identifying a protein?
The interaction that occurs between non polar molecules in an aqueous environment.
What is hydrophobic interaction?
The form of substrate that is best fit to the site of an enzyme
What is the intermediate form?
The amount of positive and negative amino acids, respectively
What is three and two?
The equation that describes initial velocity of an enzyme turning substrate into product (assuming MM kinetics)
What is V0=Vmax[S]/Km + [S]?
The instrument used to identify the photons of an amino acid.
What is a spectrophotometer?
The strongest type of bonds.
What is a covalent bond?
The units on the y-axis of a Lineweaver-Burk Plot
What is seconds per molar?
The amount of essential amino acids in our body
What is nine?
What Km represents
What is enzyme affinity to substrate?
The purification protein process that finds the n terminal amino acid of an amino acid sequence
What is Edman Degradation?
What is two uncharged atoms coming very close together?
The three reversible inhibitors
What is competitive, uncompetitive, and noncompetitive?
The amino acid that hydrogen bonds to the oxygen in myoglobin
What is histidine?
The Henderson–Hasselbalch equation.
What is pH = pKa + log([A-/HA])?
The amount of times Edman degradation can be used on an amino acid sequence.
What is 40 times?
The bond that occurs between amino acids.
What is a peptide bond?
The most common enzyme in the world
What is RuBisCo?
The amino acid that contains four carbons and two nitrogens in its R group.
What is histidine?
The initial velocity equation of a noncompetitive inhibitor.
What is V0=((Vmax/alpha)[S])/(Km+[S])?
The full names of the two reducing agents that can remove disulfide bonds.
What is Bmercaptoethanol and Dithiothreitol?
The interaction that can occur between lysine and aspartate.
What is a salt bridge?