A 10 month old who is otherwise healthy has had emesis with intermittent periods of intense crying, and is passing red stool. Which of the following actions should be done FIRST?
A. order a complete blood count
B. obtain an abdominal ultrasound
C. obtain an abdominal radiograph
D. schedule surgical evaluation for the next day
B. obtain an abdominal ultrasound
What is immunofluorescence?
A laboratory technique that uses fluorescently labeled antibodies to detect and visualize specific proteins or antigens in biological samples (and OH YA Natalie knows how to do this too)
What age group and percentage of children are affected by the preschool viral wheeze? All or nothing!
Ages 1-5, about 50% of children by age 6
What is the name of the school Natalie and Laney both attended?
University of Colorado Anschutz Medical Campus (THEY SMART SMART)
What is the major dose-limiting side effect of nanoparticle drug delivery?
a) They are extremely hard to metabolize so they accumulate in your buttock
b) They accumulate in your organs leading to intolerable toxicities
c) They are extremely polar chemical entities leading to DNA damage
b) They accumulate in your organs leading to intolerable toxicities (This was Natalie's thesis topic!)
An acute care pediatric nurse practitioner’s new oncology service role will include lumbar punctures. The legal authority to perform this task is BEST determined by:
A. obtaining credentials through board certification
B. state board’s scope of practice for nurse practitioners
C. collaborating physician's determination of practitioner’s skills
D. preparation obtained from the acute care educational program
B.state board’s scope of practice for nurse practitioners (OH and Laney passed this! SHE SMART SMART)
What is PEGylated liposomal doxorubicin?
Also called Doxil, a chemotherapeutic drug (Natalie focused on this drug for her thesis!)
List THREE of the main symptoms of preschool viral wheeze.
Coughing, runny nose, fatigue, wheezing, difficulty breathing, tight chest, sore tummy :( +100 points if you had it as a child!!!
Dress up a teammate in the cap and gown! Put the tassel on the correct side for a Master's Degree.
Tassel goes on the LEFT!!!!
What is Western Blotting?
a) A laboratory technique used to detect and analyze specific proteins in a complex biological sample
b) Cell blotting with multiple genes to create a mutant beast
c) A method used to sequence DNA by separating nucleotides based on their electrical charge
a) A laboratory technique used to detect and analyze specific proteins in a complex biological sample (and OH YA Natalie does this)
Which of the following medications has the GREATEST risk for medication-induced pancreatitis?
A. enalapril
B. quetiapine
C. valproic acid
D. vancomycin
C (but check with Laney)
Define Corticosteroids:
a) Similarly to muscle-building steroids, used to strengthen organs
b) anti-inflammatory, calms your immune system
c) a steroid that targets brain development
B!!!! Laney wrote a whole poster and presentation about this
What are the adverse effects of corticosteroids (commonly used to treat PVW)?
behavioral changes, growth suppression, immune suppression, oh no!
What is the name of Laney's degree?
Primary Care Pediatric Nurse Practitioner (Ask her!!! she's saving the world)
What the helly is an interferon lambda (IFN-y)?
a) A type of cytokine involved in antiviral immune responses
b) A bacterial enzyme that breaks down interferon proteins to evade the immune system
c) A structural protein found in the cell membrane that facilitates ion transport
a) A type of cytokine involved in antiviral immune responses (This was also Natalie's thesis focus!)
An adolescent presents with agitation, tachycardia, hypertension, hyperthermia, mydriasis, and dry, flushed skin. Ingestion of which of the following medication classes is MOST likely?
A. anticholinergic
B. cholinergic
C. opiate
D. sympathomimetic
A. anticholinergic
Literally just pronounce gastroesophageal (Laney to judge!)
I literally don't know but Laney knows!
Should pediatric nurse practitioners prescribe oral corticosteroids for preschoolers with a mild to moderate viral wheeze?
NO! Obviously it shows limited, short-term benefit but likely no clinically meaningful benefit or long-term benefit. DUH!
What is the name of Natalie's degree?
Master of Science in Pharmaceutical Sciences
What is the main benefit of interferon lambda pretreatment prior to nanoparticle administration?
a) Increases interferon lambda concentration
b) Reduces off-target accumulation of nanoparticles
c) Prevents nanoparticles from entering target tissues entirely
b) Reduces off-target accumulation of nanoparticles (Natalie studied the mechanism of action of interferon lambda for her thesis project!)
A toddler presents with bruising over multiple body surfaces including arms, legs, and trunk. Faint petechiae are also noted. Results of the CBC are provided below.
The MOST appropriate next step in management is to:
A. transfuse packed red blood cells (pRBCs).
B. administer high-dose corticosteroids.
C. order PT and PTT.
D. consult oncology.
C. order PT and PTT. (we think)
Uhhh...palmar-plantar erythrodysesthesia? Just try to pronounce this (Natalie to judge!)
I literally don't know how to pronounce this, but Natalie knows!
Your team is a pediatric nurse practitioner. A preschool-age child presents with preschool viral wheeze. They have moderate-severe symptoms, presenting with asthma, and have recurrent wheeze. They are considered high-risk. How will you treat this patient? Will you consider oral corticosteroids???
Maybe yes?? Laney judges!!
What was Natalie's specialty track for her master's program? Choose from the following:
1. Cannabis Science and Medicine
2. Pharmacokinetics and Pharmacodynamics
3. Drug Discovery
4. Molecular and Systems Toxicology
5. Biotechnology and Drug Delivery
5. Biotechnology and Drug Delivery (OH she smart smart)
Name the laboratory technique used:
What is Western Blotting (Western blot was stolen from Natalie's poster)