MOLECULAR MACHINERY
This enzyme unwinds DNA during replication.
Helicase
This level of the hierarchy of controls removes the hazard completely.
Elimination
Which pipette is best for transferring 8 µL?
P20
DNA shows peak absorbance at this wavelength.
260 nm
DNA moves toward this electrode during electrophoresis.
Positive electrode
This enzyme adds RNA primers to the lagging strand.
Primase
Gloves and goggles are examples of this type of protection.
PPE
Which pipette should be used for 950 µL?
P1000
Proteins show peak absorbance at this wavelength.
280 nm
DNA moves in the same direction because
DNA has a negative charge and is attracted to positive end
This enzyme joins Okazaki fragments together.
Ligase
A UV shield is an example of this level of control.
Engineering
Convert 1:100 into scientific notation.
10-2
OD600 is commonly used to measure this.
cell culture density
These DNA fragments travel farthest through the gel.
Smallest fragments
This structure assembles proteins from mRNA.
ribosomes
What is the most effective level of the safety hierarchy and why?
Elimination because it removes the hazard instead of just reducing exposure
A 1.2 mg/mL solution is diluted 10⁻2. What is the final concentration?
Show equation setup/work
.012 mg/mL
Measuring protein at 260 nm may produce inaccurate results because of this interference.
DNA contamination
If a gel runs too long, this may happen to the DNA bands.
Run off the gel/become hard to see
This biotechnology field compares DNA sequences using computer software.
Bioinformatics
Forgetting to document reagent lot numbers violates this laboratory principle
GLP
You need to draw up 480 uL of solution. Which pipette would you use and how would you set up the volume display?
P1000
0 4 8
Calculate the 260/280 ratio:
OD260 = 0.9
OD280 = 0.5
What does this tell you about the sample?
0.9/0.5 = 1.8
The sample is likely pure DNA
Why do smaller DNA fragments move faster through the gel?
They move more easily through gel pores