Experimental Design/Lab Math
ELISA/proteins
SDS-PAGE
Gene Therapy
PCR
100
This is what REE PE PA stands for.
What is results with evidence and explanation, possible errors, and practical applications?
100
There are this many polypeptide chains on an antibody.
What is four?
100
Polyacrylamide gels measure _______ samples than agarose gels. (bigger or smaller)
What is smaller?
100
These are the two main types of gene therapy.
What are in vivo and ex vivo?
100
This is what PCR stands for.
What is polymerase chain reaction?
200
This is the definition of a control.
What is a sample that you know the outcome of before the experiment?
200
This is the general structure of an amino acid (draw on board).
What is H H3N+-C-COO- R
200
These are two things we must do to a protein sample before running them on an SDS PAGE gel.
What is linearize and add negative charges?
200
This is the term for something used to transmit DNA from one organism to another.
What is a vector?
200
This is the step where primers bind.
What is annealing?
300
These are three things required for a valid experiment.
What are controls, one variable, a testable hypothesis, etc.
300
This is the term for a protein or small molecule that an antibody binds to.
What is an antigen?
300
Proteins separate on a gel based on this.
What is MW?
300
These are two alternatives to vectors for use in gene therapy.
What are naked DNA, gene gun, liposome, etc?
300
These are the there steps of PCR, in order.
What is denaturing, annealing, and extension?
400
This is how many uL of a 30x solution you would add to make 150 mL of a 2x solution.
What is 10,000 uL?
400
This is the main difference between a direct and an indirect ELISA.
What is an extra antibody binding step (enzyme linked to secondary antibody instead of primary)?
400
This is what SDS-PAGE stands for.
What is sodium dodecylsulfate polyacrylamide gel electrophoresis?
400
This is the type of virus used most often for permanent integration, and this is the vector used most often for precise placement in a genome.
What is retrovirus and adeno-associated?
400
When genotyping, this is the type of DNA you want to focus on.
What is highly variable?
500
How many g of NaCl would you add to make 350 mL of a 0.5 M solution? MW of NaCl is 58.5.
What is 10.2g?
500
This is a brief description of how an ELISA works (think of the lab, make sure you include each step).
What is sample placed in well, any antibodies present in sample will bind to antigens in well. Wash off any unbound antibodies. Secondary antibody has enzyme attached, it will bind to any bound primary antibodies. Wash off any unbound. Add substrate, if enzyme is present, then substrate will change color.
500
This is a brief description of how you would create a standard curve.
What is run a MW marker, plot the distance travelled by each band on the X axis and known MW on the y-axis, find a line of best fit through the points.
500
These are three issues associated with the use of gene therapy in patients.
What are immune response, targeting, permanency, etc?
500
These are all of the components of a PCR reaction.
What is template DNA, primers, Taq polymerase, dNTPs, buffer?
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