MICROBES
GENETIC ENGINEERING
INDUSTRIAL BIOTECH
MEDICAL MICROBES
ENV & AG BIOTECH
100

Unlike eukaryotes, these single-celled organisms lack a membrane-bound nucleus.

Prokaryotes (Bacteria / Archaea)

100

Enzymes that act as molecular scissors by cutting DNA at specific recognition sequences.

Restriction Enzymes

100

Saccharomyces cerevisiae, commonly known as baker's or brewer's yeast, converts sugars into carbon dioxide and this alcohol.

Ethanol

100

Discovered by Alexander Fleming in 1928, this was the first widely used natural antibiotic.

Penicillin

100

The process of using living microbes to clean up pollutants, oil spills, or toxic waste from soil and water.

Bioremediation

200

This protein coat wraps around a virus's genetic material to protect it.

Capsid

200

Small, circular loops of double-stranded DNA commonly used as vectors in cloning.

Plasmids

200

Large vessel used to grow microorganisms under controlled temperature, pH, and oxygen conditions for product harvest.

Bioreactor (or Fermenter)

200

Instead of whole pathogens, lipid nanoparticles deliver this genetic blueprint into human host cells in modern COVID-19 vaccines.

mRNA (messenger RNA)

200

Symbiotic bacteria of the genus Rhizobium live in root nodules of legumes and perform this vital atmospheric process.

Nitrogen Fixation

300

Fungal cell walls are mainly made of this tough structural polysaccharide, also found in insect exoskeletons.

Chitin

300

This lab technique uses cycles of heating and cooling to amplify targeted DNA sequences millions of times.

PCR (Polymerase Chain Reaction)

300

Biodegradable polymers such as PHA (polyhydroxyalkanoates) produced naturally by microbes as carbon storage are called these.

Bioplastics

300

Identical antibodies cloned from a single hybridoma cell line, designed to target specific antigens in cancer therapy.

Monoclonal Antibodies

300

This soil bacterium produces crystalline Bt proteins widely engineered into crops as natural insect repellents.

Bacillus thuringiensis (Bt)

400

Unlike bacteria, these domain members have ether-linked membrane lipids and thrive in extreme environments.

Archaea

400

In the CRISPR-Cas9 system, this molecule directs the Cas9 nuclease enzyme to the exact target DNA sequence.

Guide RNA

400

Enzymes from microbes added to laundry detergents to break down tough oil and lipid stains.

Lipases

400

This rapid molecular diagnostic test uses antibodies linked to enzymes to detect antigens or antibodies in a sample.

ELISA (Enzyme-Linked Immunosorbent Assay)

400

Organisms that thrive in high-salt habitats, such as Halobacterium salinarum, are classified under this extremophile type.

Halophiles

500

The process by which a bacterial cell directly transfers genetic material to another through a pilus.

Conjugation

500

This enzyme connects Okazaki fragments or recombines cut DNA fragments by forming phosphodiester bonds.

DNA Ligase

500

A fermentation system where nutrients are continuously added and waste/product is continuously removed to maintain exponential growth.

Continuous (Chemostat) Fermentation

500

Bacteriophages are specialized viruses used in therapy to target and kill these specific pathogens.

Bacteria (Bacteriophage / Phage Therapy)

500

Microbial fuel cells convert chemical energy stored in organic compounds directly into electricity using these biological catalysts.

Electroactive Microbes (Electrochemically Active Bacteria)

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