DNA/RNA Organization
Would the pattern of gene expression in the liver cell of person #1 be more similar to the heart cell of person #1 or the liver cell of person #2.
More similar to the liver cell of person #2
What causes the molecules to migrate down a gel during electrophoresis.
The current applied by a power source
A sample of normal double stranded DNA was found to have a guanine [G] content of 12%. What is the expected proportion of adenine [A]?
38%
In a prokaryote, is a transcription factor that prevents the binding of the protein sigma, an example of positive or negative regulation
This would be negative regulation
When designing a hybridization probe for northern or southern blotting, do you want the sequence of the probe to match the target DNA sequence or be complementary to the target DNA sequence
You want the probe to be complementary to the target DNA sequence
At a specific point in the human genome, an Adenine (A) is found in 3.2% of the population. Would this be considered a point mutation or a SNP (single nucleotide polymorphism)
List three levels of regulating gene expression in Eukaryotes
Any three from:
Chromatin/DNA accessibility, transcription initiation, RNA splicing/processing, RNA stability, translation control by miRNA/siRNA, or post-translational modification
Which of the following techniques would you use to measure the concentration of a target RNA sequence?
Northern, Southern, or Western blot?
Northern blot
What is the cellular location and function of the DICER protein?
DICER is located in the cytoplasm of the cell and is involved in the cleaving and processing of pre-miRNA into miRNA:miRNA (double stranded)
You perform a western blot to analyze the size of a target protein in two samples: WT and mutant. If the mutant protein had a missense mutation, would you expect to find the band above, below, or on the same level as the WT protein band.
You would find it on the same level as the WT band
Which gene(s) (Z/Y) will a cell with this Lac Operon genotype be able to express in the presence of lactose (induced):
I+ P+ OC Z- Y+ / IS P+ O+ Z+ Y-
In the presence of lactose (induced), this cell can only produce gene Y
When analyzing STRs (short tandem repeats) from a parent and a child on a gel, you notice the parent shows one band and the child has two bands, one at the same level as the parent, and another higher up.
Does the other parent (not analyzed) need a longer or shorter STR than the parent who was analyzed.
The other parent needs a longer STR