Single and Ready to Mingle
This term describes the process by which single progenitor cells commit and differentiate into cell types with a restricted lineage.
What is a developmental trajectory?
These two types of bonds are necessary to form a double stranded DNA molecule.
What are phosphodiester and hydrogen?
In Illumina sequencing, this step results in clusters containing hundreds of identical
strands of DNA in millions of unique locations across the flow cell surface at the same time.
What is bridge amplification?
There are this number of colors that are used to interpret which nucleotides are part of a sequence in both Illumina and Pacific Biosciences sequencing.
What is four?
RNA-seq data follows this type of distribution characterized by a long right sided tail.
What is a negative binomial distribution?
This can be defined by morphology, location, molecular markers, ontogeny, their transcriptome, and function.
What is a cell type?
The product of the reverse transcriptase enzyme and an mRNA template.
What is cDNA?
This oligonucleotide sequence enables the sequencing of many different RNA-seq libraries together in a lane of a single flow cell.
What are barcodes or indices?
The fluorophores used in Illumina sequencing are attached to this structure.
What is the residual linker structure?
On this type of graph, color represents gene expression level.
What is a heat map?
This algorithm allows scientists to group cells based on their transcriptome similarities and dissimilarities.
What is t-SNE?
The process that occurs as soon as an mRNA is transcribed, resulting in the addition of a 3' polyA tail.
What is co-transcriptional processing?
This type of sequencing has a high error rate, but it is random.
What is Pacific Biosciences Sequencing?
In microarray analyses, the expression level of a gene is measured by the ________ of signal from perfect match and mismatch probes; relative to the levels of all probes on a microarray chip.
What is relative intensity?
This type of analysis groups samples in multidimensional space by maximizing variation between groups, and minimizing variation within a group.
What is clustering?
This type of gene can serve to differentiate a specific cellular cluster from another:
What is a marker gene?
This functional group on the sugar molecule differentiates RNA from DNA.
What is the 2' hydroxyl group?
This mode of Illumina sequencing results in at least 100 bp sequencing reads.
What is paired end?
This is the name for the special type of nucleotides used in Pacific Biosciences sequencing.
What is phospho-linked?
These are the three types of Gene Ontology terms.
What are Biological Function, Molecular Process, and Cellular Component?
The number of cells sequenced using single cell transcriptome sequencing is identified using this oligonucleotide label.
What is a cell-specific label?
The base pairing that is the most difficult to separate due to its hydrogen bonds.
What is C-G?
Pacific Biosciences sequencing utilizes phospho-linked nucleotides, zero mode waveguide, and this enzyme to generate sequence data.
What is the pause polymerase?
In single cell transcriptome sequencing, one should perform this type of analysis, with a visual reporter, to confirm that additional cell clusters represent real cell populations.
What is validation of a cell type?
This type of plot allows one to look at the median, quartile range, and the distribution of all data points.
What is a violin plot?