Oh Baby!
what is the most common cause of HDFN?
ABO incompatibility
what is the immundominant sugar for A antigen formation?
n-acetylgalactosamine
What does a snowman like to eat for breakfast?
Frosted flakes
How often do we do reagent QC in Blood bank?
at least once a day
what did Ralphie want for Christmas in A Christmas Story?
Red Ryder air rifle BB gun
A mother is found to have anti-Fya The father is Fy(a-b+)
Is the baby at risk for HDFN?
no. The baby will not inherit Fya
What % of group A individuals are A subgroups?
20%
Why do we add enhancement to tube antibody screen?
reduce zeta potential, allow agglutination
Give an example of phenotype of cells you would choose for phenotyping for E
E+e+
what is the spinning top game that children play on Hanukkah?
dreidel
why do we wash cord blood samples thoroughly before testing?
to remove whartons jelly
a patients red cell react with anti-B, and the serum reacts with group A cell. What is their blood type?
B
Name an HLA gene that is not in the MHC Class I region
Class I HLA region contains genes for HLA-A, -B, and -C
Class II HLA region contains genes for HLA-DR, DP, and DQ loci
When do we need to do a saline RHD QC for tube typing?
AB Pos
In the song “Frosty the Snowman,” what made Frosty come to life?
an old silk hat
a newborn has a 4+ DAT caused by maternal anti-D. The mother is O dce/dce with anti-D. The father is A, R1R1.
The infant types as O neg. What is causing the discrepancy?
(Explain what is meant by 'blocked D')
maternal antibody is bound to fetal D antigen sites and prevents anti-D typing reagent access to fetch D antigen sites. Results in false negative result.
Blocked D
What is the likely cause of this ABO discrepancy?
Anti-A 3+
Anti-B 0
A1 cells 0
B cells 0
old age
Deletion of Glycophorin B gene results in no Glycophrin B. Which antigens would the individual be lacking? In what population is this most prevalent?
U,S,s
Ena phenotype has no GPA , no M, N
Mk phenotype is M null. No MNS blood group antigens. M,N,S,s, En, Wr
The most widely accepted QC test to measure probable Hematopoietic Progenitor Cell (HPC) engraftment is
what is the name of the Grinch's dog?
Max
Name 4 requirements for blood for intrauterine transfusion
Antigen Negative for Mom’s corresponding antibody
AHG compatible with mother serum
Usually group O Rh negative
CMV Negative (or CMV ‘safe’-leukopoor)
Irradiated to prevent GVHD in immunologically immature fetus
Freshest Available (<7 days old)
Hgb S negative – recommended to prevent icklingunderlow O2 tension
you are typing a person who you expect is a Bombay phenotype. To confirm you use a lectin. What lectin?
If the patient is a Bombay Patient cell's + lectin = ?
(agglutination or no agglutination)
ulex europaeus
agglutination!
Which is most likely responsible for an incompatible IAT crossmatch?
a. Recipient's RBCs possess a low frequency antigen
b. anti-K in donor serum
c. recipient's RBCs are polyagglutinable
d. donor RBCs have a positive DAT
donor RBCs have a positive DAT
according to AABB standards, at least 90% of platelets prepared from whole blood shall have at least how many platelets?
a. 5.5 x 1010
b. 6.5 x 1010
c. 3.0 x 1011
d. 5.0 x 1011
a.5.5 x 1010
for apheresis 3.0 x 1011
(others: Hct RBCs , granulocytes, plt pH at end of storage min 6.2)
Name Santa's 8 reindeer
Dasher, Dancer, Prancer, Vixen, Comet, Cupid and Donner , Blitzen
(and Rudolf and Olive!)