APP Processimg
What does APP stands for?
Amyloid Precursor Protein
Who discovered AD?
Dr. Alzheimer
What is the name of the cell culture-specific hood?
Biosafety cabinet
What formula used to calculate stock volume needed for antibody dilutions?
C1V1=C2V2
Where did the hippocampus get its name from?
A seahorse
What is synthesized when b-CTF is cleaved by y-secretase?
Amyloid-b
What are the two types of AD?
Sporadic and familial AD
Name 2 things found in cell media
phenol red, buffer, nutrients, vitamins, minerals, nucleic acids...
What is the process used to separate proteins/DNA by size?
gel electrophoresis
What disease was studied by Liz before she studied AD?
CF
What enzyme is responsible for amyloid-B degradation?
ECE
What are the 2 main AD pathologies (causes) in the brain?
Ab plaques and Tau tangles
How does the MTT assay asesses cell viability?
mitochondrial activity
What step is needed to prevent non-specific binding in ELISA and WB?
blocking
What dye was used to stain plaques when performing IF?
Congo Red
What enzyme cleaves APP to form b-CTF?
b-secretase
What forms on the extracellular part of neurons during AD?
Ab plaques
What is the name of the device used to count cells?
Hemocytometer
Name a compatible secondary antibody if my primary ab is as follows:
C-term APP - Rabbit
goat anti-rabbit (or donkey anti-rabbit)
What amount of stock antibody is needed to make a 1:3000 dilution of ab, in 15ml final volume?
5uL
What enzyme cleaves APP to form a-CTF?
a-secretase
What is the current treatment of AD?
helping Ab plaques breakdown
What is the compound/protein used to detach cells from flask prior to seeding?
Trypsin
What part of the detection antibody reacts with TMB substrate to create color during an ELISA?
HRP-conjugated antibody
What is the wavelength used to detect blue or yellow color on spectrophotometer (absorbance).
650 (blue) and 450 (yellow)