True or False: Enzymes are used up during their reaction.
False: Enzymes do not get used up. They are free to react again and again. This is why they are only need in the body in small amounts.
What region contains the optimum temperature for this enzyme?

III (3)
Which number represents the substrate?

1
How do cold temperatures affect the rate of reactions for enzymes?
Decreases / slows down
What is the thing the Enzyme acts upon?
Substrate
How do enzymes affect the speed of reactions in the cells?
Increase speed

At what pH is Urease most effective?
7
What is our Biology Room Number
How does increasing substrate concentration affect the rate of an enzymatic reaction?
The reaction rate increases until the enzymes are all used up.
What is the “new” substance created during the reaction?
Product(s)
What type of macromolecule are enzymes?
Proteins
Which number represents the active site?

2
Which number represents the enzyme-substrate complex?

3
What are two factors that can change the shape of an enzyme, causing it to stop working?
Temperature and pH
Where does the substrate attach to the enzyme?
Active site
This graph is showing that enzymes _____________.

Lower the activation energy required to start the reaction
The optimal temperature and pH of this enzyme is _______.
Temp = 37C
pH = 6
What subject are you going to learn next school year?
Chemistry
How does a non-competitive inhibitor change the function of an enzyme?
It binds to the enzyme (somewhere other that the active site), which changes the shape of the active site, causing the substrate to no longer fit.
Enzymes lower activation energy & speed up reactions. Another word for something that does this is a ____________.
Catalyst
Explain what is meant by "Enzymes are substrate SPECIFIC". Which model would this fall under?
They only react with certain substrates that fit into the active site.
Lock and Key!
Students had this reaction at a temperature of 70C (TOO HOT) and did not see any enzyme activity. Why?

The temperature was too high and denatured the enzyme causing it not to function
What is the optimum temperature of the human body?
98.6 F
37 C
Using your knowledge of enzymes, how does an activator help the reaction occur between an enzyme and a substrate?
The activator attaches to the enzyme to help change the active site shape so the substrate can bind to the active site.
When the substrate binds to the enzyme, something called "induced fit" happens. What is induced fit?
The enzyme temporarily changes its shape at the active site. This change in shape is what causes the reaction to happen more easily.