Exam 1
Mendel's law of segregation
Where does DNA polymerase get its energy?
Incoming dNTPs (dATP, dTTP, dGTP, dCTP)
What direction are nucleotides added during transcription? What direction is the template strand read in?
Additions - 5' to 3'
Read 3' to 5'
Describe the P site of the ribosome.
It is where the polypeptide chain forms
Are mutations in tumor suppressor genes usually dominant or recessive?
Recessive
Which law states that the alleles on two different genes get sorted without influence from one another?
Mendel's law of independent assortment
Where would you find the radioactive sulfur if you replicated the Hershey-Chase experiment?
Outside the pellet in the phage coats
Binds to the core promoter and begins assembly of the transcription complex
What type of methionine and tRNA are used to initiate translation in prokaryotes?
f-met and initiator tRNA
What is the 68-95-99 rule?
68% between 1SD, 95% between 2SD, 99% between 3SD
If the trait pictured in this pedigree is rare, what is the inheritance pattern and what is the affected mother's genotype? (go to powerpoint for picture)
RNA primer
What makes up RISC and where does RISC bind?
Composition - miRNA or siRNA and Argonaute
Binds to 3' UTR (untranslated region)
(see powerpoint for picture)
What is the reverse primer for the following sequence: 5' ATCGTT------------------------TGCGTA 3'
5' TACGCA 3'
What is the H-W expected frequency for Aa given the following allele frequencies?
A - 0.35
a - 0.65
0.455
What is the range of phases that contain sister chromatids in meiosis? What range contains homologous chromosomes?
Sister chromatids - Prophase I through metaphase II
Homologous chromosomes - Prophase I through metaphase I
What percent of the offspring in a testcross of brittle stock and white endosperm cap would be recombinant? (go to powerpoint for picture)
25%
The lac operon is inducible. How is the lac operon turned on?
Cofactor binds to repressor protein -> repressor protein unbinds from the operator
What is the order of the enzymes given this supplementation data? (go to powerpoint for picture)
E3, E1, E2
What is the inbreeding coefficient given this data? (go to powerpoint for picture)
0.25
Which of Mendel's laws does gene linkage break?
Mendel's law of independent assortment
What is the map distance between a and b? (go to powerpoint for picture)
9.6 cM
The repressible trp operon has a mutation so that the cofactor cannot bind to the repressor protein. What is the state of the operon?
Always on
You have two groups of mice. One group has a recessive mutation for purple toes instead of pink toes. The other group has a recessive mutation for large ears instead of small ears. If you cross a purple toed mouse with a large eared mouse, what would the offspring phenotypes be?
Pink toed and small eared
0.56