DNA Replication
This enzyme unwinds the DNA double helix at a replication fork.
Helicase
This enzyme makes RNA from a DNA template.
RNA Polymerase
This three-base sequence usually starts translation.
AUG
This protein binds an operator and inhibits transcription.
Repressor
This enzyme makes the RNA primers needed for DNA synthesis.
Primase
This factor helps bacterial RNA polymerase recognize a promoter.
Sigma factor
This sequence helps the bacterial 30S subunit bind mRNA in the correct position.
What is the Shine–Dalgarno sequence
This molecule binds CRP so CRP can activate lac transcription.
cAMP
This enzyme replaces RNA primers with DNA on the lagging strand.
DNA polymerase
A single mRNA encoding proteins from multiple genes is called this.
Polycistronic mRNA
An incoming charged tRNA first enters this ribosomal site.
P-site
End product of the pathway binds to an early (often the first) enzyme in the pathway
What is feedback inhibition
DNA synthesis occurs continuously on this strand and in fragments on the other.
Leading Strand
This RNA structure can cause RNA polymerase to pause and terminate transcription.
Stem loop
This enzyme attaches the correct amino acid to its tRNA.
aminoacyl-tRNA synthetase
When lactose is present and glucose is absent, this is the inducer
Allolactose
DNA polymerase adds nucleotides from the
5' -> 3'
The consensus sequences on the DNA promoter are
-35 and -10
A mutation disrupts pairing between the Shine–Dalgarno sequence and 16S rRNA. This step is most directly affected.
Lactose is present, but glucose is also present. This explains why lac transcription remains relatively low.
ow cAMP, resulting in little CRP activation