Reference Ranges
This is the normal WBC range for adults.
4.8–10.8 x10³/µL
This is the ideal angle for a good smear.
30–45°
How many WBCs are counted for a differential?
100 WBCs
This test detects hemoglobin S.
Sickledex
First recognizable WBC stage.
Myeloblast
Normal platelet range.
150,000–450,000/µL
Too steep an angle produces this type of smear.
Thick smear (>45°)
How many fields are used for WBC estimation?
10 HPFs
Positive Sickledex result looks like this.
Opaque (can’t see lines)
Last stage capable of mitosis.
Myelocyte
Normal neutrophil percentage.
50–70%
This reagent fixes the sample to the slide.
Methanol
This power is used to evaluate stain quality and distribution.
10x (low power)
This test detects heterophile antibodies.
Monospot
This stage has a kidney-shaped nucleus.
Metamyelocyte
Normal lymphocyte percentage.
20–44%
This stain colors hemoglobin and cytoplasm pink/red.
Eosin
This area is best for morphology evaluation.
Monolayer (before feathered edge)
Positive Monospot result shows this color line.
Blue line
This stage has a horseshoe-shaped nucleus.
Band neutrophil
Normal MCV range.
80–100 fL
This stain colors nucleus blue/purple.
Methylene blue
List the 3 things evaluated under low power.
Stain quality, Cell distribution, Optimal area
This stain detects myeloid cells via primary granules.
Myeloperoxidase (MPO)
List all granulocyte maturation stages.
Myeloblast → Promyelocyte → Myelocyte → Metamyelocyte → Band → Segmented