Project Goals
The type of animal used in whole brain proteomics profiling
What is a mouse?
The number of antibodies used in my project
What is 10?
The gel used in expansion microscopy
What is hydrogel?
Through amplification, the number of fluorophores on each barcode...
What is increases?
The research that won the Nobel Prize for physiology and medicine
What is optogenetics?
The goal number of antibodies in our finished proteomic atlas
What is 100?
The type of antibody used in my project
What is neuropeptide expressed NeuN (Neuronal Nuclei; RBFOX3), Calretinin (CALB2), GFAP (Glial Fibrillary Acidic Protein), SST (Somatostatin), ALDH1L1 (Aldehyde Dehydrogenase 1 Family Member L1), NPY (Neuropeptide Y), PV (Parvalbumin), Calbindin (CALB1), TH, or NOS1?
The molecule that the gel is primarily composed of
What is water?
Amplification seeks to ___ barcode length
What is increase/extend?
The people that won the Nobel Prize for physiology and medicine
Who are Karl Deisseroth, Peter Hegemann, and Georg Nagel?
The name of the technique used to map out individual neural circuits
What is brainbow?
The brain section used in my panel
What is (left hemisphere) medial sagittal section?
The reason gel expansion microscopy is used
What is allowing imagers to freely diffuse and bind their, improving labeling uniformity, ensuring that densely packed molecular targets can be detected simultaneously across the tissue, or enhancing imaging by reducing light scattering through tissue?
The reason why long DNA barcodes are not initially used
What are accessibility and binding issues?
The Nobel Prize corresponding to the discovery of GFP (green fluorescent protein)
What is the 2008 Nobel Prize in Chemistry?
The mechanism that refers to specific hydrogen pairing between nitrogenous bases
What is Watson-Crick base pairing?
The technique used in our project for antibody validation
What is secondary-antibody-only control?
The method used to clear tissue in our project
What is SDS clearing?
The amplification method used in my experiment
What is INSERT?
The animal corresponding to the discovery of GFP
What is the jellyfish Aequorea Victoria?
The label attached to antibodies that allows fluorophore-labeled DNA oligos to specifically find one type of antibody
What is DNA Barcode?
Technique used to reveal specificity of antibody staining
What is DAPI staining?
The more aggressive digestion-based clearing method
What is ProK (proteinase K) -based clearing?
The method where strand-displacing polymerase continuously extends the barcode while simultaneously pushing off the previous template
What is INSERT?
The female Nobel Prize winner for click chemistry
Who is the goat Carolyn Bertozzi?