DIY DLI
Rhizosphere Microbiology
Go With the Flow
Macro Nutrients by Element
Micro Nutrients by Element
100

List the equation to calculate DLI.

100 Bonus: What does DLI stand for? 

((PPFD x 3600) x photoperiod) / 1,000,000


100 Bonus: Daily Light Interval

100

Describe what the rhizosphere is.


100 Bonus: Describe the phyllosphere.

The rhizosphere is the part of the plant that is below the surface of the substrate. 


100 Bonus: The phyllosphere is the part of the plant above the surface of the substrate.

100

Name two types of hydroponic irrigation systems. 

100 Bonus: What type do we use in our farms? 

Ebb and flow, NFT, deep water, shallow water, aeroponics, Dutch bucket, drip, Kratky

100 Bonus: Ebb and Flow

100

This element is the most essential for plant growth and is the only gaseous nutrient that we supplement. 

100 Bonus: Name the compound of that element that is added to our grow zone.  

Carbon 


100 Bonus: Carbon dioxide

100

Name the mineral macronutrient that is needed in the greatest volume by the plant. It is the micronutrient that is most easily dropped out of solution by UV and Ozone. 

100 Bonus: Describe the visual symptoms of its deficiency. 

200 Bonus: Why? 

Iron 


100 Bonus: Chlorosis of new growth with uniform coloration and steady transition. 

200 Bonus: Iron is used to create the precursor heme proteins later used in the creation of chlorophyll, but iron does not end up in chlorophyll itself. 

200

What is your DLI if your photoperiod is 18 hours and the PPFD is 250 μmol?   

16.2

200

Plants can signal the microbes in the rhizosphere using what substance? 

The composition of this substance can change depending on the life stage of the plant, stress, and the mineral nutrient deficiencies the plant is experiencing. 

Root exudates 
200

We typically measure water volume in cubic meters instead of gallons. 

How many liters are in a cubic meter? 

100 Bonus: How many gallons are in a cubic meter?

1 cubic meter is 1,000 litres


100 Bonus: 1 cubic meter is 264.17 gallons 

200

This element is the most numerous of the mineral nutrients within the plant. It makes up to 20% of the total biomass of a plant by constituting the majority of the building blocks for proteins, nucleic acids, etc. It is important to balance this nutrient with potassium to steer plant growth.

100 Bonus: Name one of compounds of that element that is added to our grow zone.    

Nitrogen

100 Bonus: Ammonium, Nitrate, CaNO3, KNO3, NH4NO3, MgNO3

200

This mineral micronutrient is the one that plants need the smallest volume of in the nutrient solution. It is needed for the metabolism of ammonium, along with other enzyme functions. 

100 Bonus: It is the densest nutrient we add, what is its atomic number? 

Molybdenum 

100 Bonus: 42

300

What is your DLI if your photoperiod is 20 hours and the PPFD is 370 μmol?  

26.64

300

True or False: Any species of bacteria or fungus can for symbiosis with plants. 


100 Bonus: Name two species of beneficial microbes we use for our crops. 

False: The symbiotes must be able to consume the exudates that are given to it by the plants, and provide benefits to the plant itself. Technically all saprotrophs help the plant by liberating nutrients, but not in close association with the plant itself. 

100 Bonus: Bacillus subtillis, Trichoderma hazarium, Trichoderma viridens, Pseudommonas, Geobacillus, Sphingommonas, Glomus, etc. 

300

Pressure is a force on a fluid given per unit area. 

True or False: The same amount of force applied to a smaller diameter pipe of water creates a greater pressure than the same force applied to a larger diameter pipe. 

200 Bonus: Do we use Kpa or PSI in most of our measurements? How many Kpa = 1 PSI? 

True: Same amount of force in a smaller pipe would create a greater pressure than the same force in a larger pipe since the pressure is determined by unit area. 

200 Bonus: Kpa should be standard, 1PSI = 6.9Kpa

300

This element is important for the formation of phospholipids, ATP, and is typically limited in the natural environment. 

100 Bonus: Name the compound of that element that is added to our grow zone.  

Phosphorus

100 Bonus: Monopotassium Phosphate, Phosphate

300

This element is an essential micronutrient that is important for many things, but especially for root growth and elongation. 

100 Bonus: It is the second most dense element we add to the nutrient solution. What is its atomic number? 

Zinc


100 Bonus: 40 

400

What is your DLI if your photoperiod is 22 hours and the PPFD is 186 μmol?  

14.7

400

True or False: Brassicas are famously known to not form symbiotic relationships with endosymbiotic plant growth promoting microbes. 

False: Brassicas do not form those symbiotic relationships. 

400

What is it called when there is a more viscous part of gas or liquid near the surface of a solid object that creates a velocity gradient. 

100 Bonus: Why does it matter to us?

Boundary layer


100 Bonus: It is particularly important for us since the dryer air we are passing over the plants may not come in close contact with the plants due to the more humid boundary layer of air surrounding the leaves. 

400

This element is important because it forms essential enzymes and the plant uses it to control osmoregulation through the roots and the stomata's guard cells. 

100 Bonus: Name the compound of that element that is added to our grow zone.  

Potassium 

100 Bonus: Monopotassium phosphate, Potassium chloride, Potassium nitrate.


400

This non-essential but beneficial micronutrient helps fortify cell walls, increases sap flow, and promotes host defense mechanisms. What is it? 


300 Bonus: Name the two essential mineral nutrients, and one non-mineral nutrient that impact cell wall integreity the most. 

Silicon

300 Bonus: Calcium, Boron, and Carbon

500

What is your DLI if your photoperiod for 16 hours is 350 μmol, but there is a 2 hour ramp at the beginning of the photo period and a 2 hour ramp at the end of the photoperiod that has a PPFD of 175 μmol?

20.7

500

Describe the differences between ecto- and endo- mycorrhizal fungi

Ectomycorrhizal fungi grow on the surface of the roots, covering them with mycelium. (most common in trees)


Endomycorrhizal fungi grow in between root cells to form their symbiosis. (what we see most in our plants)

500

Do we aim for laminar flow or turbulent flow with the airflow in our growzones? 

100 Bonus: Why?

200 Bonus: Describe the difference between the two types of airflow.

Laminar is the goal, but difficult to achieve

100 Bonus: The energy required and the effect on the plants.

200 Bonus: Laminar airflow is when low flow or small obstacles allow air to flow in an organized way smoothly across different boundary layers. Turbulent flow is created by irregular eddies and inconsistent flow with higher speeds and larger obstacles.  

500

This element is key for the formation of chlorophyll, as well as other enzyme functions. It has an antagonistic relationship with calcium since they are very close on the periodic table. 


100 Bonus: Name the compound of that element that is added to our grow zone.  

Magnesium 


100 Bonus: Magnesium sulfate, Magnesium nitrate

500

This essential mineral micronutrient is needed in very small amounts and can be toxic at high concentrations. It is commonly used in commercial fungicide, algicide, and herbicides. It has limited mobility in plants and is most commonly found in high concentrations in the roots. What is it? 

100 Bonus: Name 2 factors that can influence the uptake of this nutrient in our irrigation water. 

Copper


100 Bonus: pH, temperature, total availability, nutrient antagonism, oxidation state: Cu+2

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