Staining
chromophore is a cation
chromophore is an anion
What is acidic dye?
gram positive color
What is purple?
gram negative color
What is pink?
stain absorbed by the cells
What is positive stain?
stain absorbed by the background
What is negative stain?
gives color to all cells
What is primary staining?
used to hold the stain or coat the specimen to enlarge it
What is mordant?
removes the purple from the peptidoglycan of some cell species but not from others
What is decolorizing agent?
application of second stain with a contrasting color to sample for microscopy
What is counterstain?
Alcohol dehydrates peptidoglycan, CV-I crystals don't leave
What is staining in gram-positive?
alcohol dissolves outer membrane leaving holes in peptidoglycan
What is staining in gram negative?
gelatinous covering that do not accept most dyes
What are capsules?
highlights cell structure
What is capsule staining?
specimen placed in liquid
What are wet mounts?
Why we need staining
What is to increase contrast?
the use of heat or chemicals for staining
What is fixation?
acid fast stain that uses heat
What is Zihel-Neelsen technique?
acid fast staining that does not use heat
What is Kinyoun technique?
acid-fast stain use
What is mycobacterium and nocardia?