mRNA parts
The part of a mRNA that specifies the polypeptide sequence
What is the coding region?
A transcriptional repressor that prevents high level expression of the lac operon in the absence of lactose
What is the Lac Repressor?
A type of DNA that is used to make sure that the binding of a transcription factor to a binding site is specific for that binding site.
What is salmon sperm DNA?
The part of a protein that recruits RNA polymerase II to a gene for transcription.
What is a transcriptional activation domain?
What is the 3'UTR?
A substrate for beta-galactosidase that forms a blue reaction product and is useful to indicate the amount of beta-galactosidase activity in a cell
What is X-Gal?
A piece of DNA in which the coding region of a gene is fused to the coding region of a gene like GFP
What is a protein reporter gene?
When 2 identical proteins bind each other to form a functional unit.
What is a homodimer?
The sequence at the very 3'end of all mRNAs
What is the polyA tail?
The transcriptional activator for the lac operon that binds DNA in low glucose conditions.
Used to separate denatured proteins by size before a Western blot
What is an SDS gel?
The part of a protein that makes sequence-specific contacts with DNA.
What is a DNA-binding domain?
A sequence of nucleotides surrounding the start codon that increases translation
What is the Kozak sequence?
The binding site for the Lac Repressor
What is the Operator?
A piece of DNA in which the regulatory region of one gene is fused to the coding region of a gene like GFP or luciferase
A sequence in a mRNA from which a protein can be translated without a stop codon
What is an open reading frame (ORF)?
The nucleotides at the very beginning of almost all coding regions of mRNA that specifies the start of translation
What is the AUG?
or What is the start codon?
What is allolactose?
Used to separate differently-sized complexes in a gel-shift experiment
What is a non-denaturing gel?
The concentration of the reactants multiplied together divided by the concentration of the reaction product.
What is Kd?