Cell Structure
Name the two proposed models for cell wall of RT761?
S layer + OM + CM
AND
OM + CM + Nucleoid (intracytoplasmic) membrane
What percentage of genes in RT761 bacteria contain transmembrane helices?
~30%
What are the fermentation end products (at least 2) of RT761 from glucose?
Acetate, Hydrogen, CO2, Ethanol
How long was the enrichment process of saline water and sediments that resulted in RT761 isolate?
3 years
What are the two microcopy techniques used to determine that the inner layer encapsulates the nucleoid?
Confocal fluorescence and TEM
What was the proposed function of NTEs?
Metabolic function (encapsulation of enzymes/intermediates with BMC)
Why do RT761 rely on syntrophic interactions for ideal growth and with what other species?
reduce the concentration of hydrogen in the environment.
syntrophic interactions with methanogenic archaeon
Atribacter laminatus
What are all 5 microscopy techniques that were shown and utilized in this paper?
Phase contrast
Cryo-ET
Confocal fluorescence
TEM
SEM
identification of bacterial lineages with unique cell membrane structure
How does the RT761 strain differ from other sugar-degrading bacteria that benefit from syntrophic interactions?
RT761 does not produce lactate as a fermentation end product and it does not contain NiFe hydrogenases.
It rather contains energy conserving complexes such as the Rnf Complex and trimeric/tetrameric FeFe hydrogenases.
What are the 4 reasons presented that makes bacteria "unculturable"?
2. VBNC state
3. Stringent Requirements
4. Co-dependence on other Microbes