Bacteria Structure
Used to translate genetic material into proteins
Ribosome
Stain that yields a positive or negative result based on the thickness of a cellular structure.
Gram staining
Technique used to eliminate contamination from pathogens
Aseptic
susceptibility test that first helps determine antibiotic resistance
Disk Diffusion
Disk Diffusion and Broth Dilution
Unbonded region where DNA is found
Nucleoid
Name the three shapes
1. Spherical
2. Rod
3. Spirals
1. Cocci
2. Bacilli
3. Spirilla
1. Introducing the specimen to a growth medium
2. Microorganisms that have grown/process of growing
1. Inoculation
2. Culture
What does a clear ring around an antibiotic disk mean in disk diffusion
The bacteria is susceptible to that antibiotic
A microorganism capable of causing disease
Pathogen
Sticky outer layer that aids in antibiotic resistance
Capsule/Glycocalyx
Secondary staining that must be used if the cell has a hydrophobic layer outside the cell wall
Acid-Fast
Media that favors some organisms while suppressing others
What does broth dilution test for?
The minimal inhibitory concentration (MIC)
Feature of this bacteria that prevent effective gram staining
Mycolic acid/Waxy envelope
Provides structure and is directly responsible for gram staining result
Cell Wall
Result given to pink bacteria past gram staining
Gram negative
Media that identifies bacteria based on reactions in the media
Differential
1. The minimum concentration of antibiotic that produces no VISIBLE growth
2. The minimum centration of antibiotic that produces NO growth
1. Minimum Inhibitory Concentration
2. Minimum Bactericidal Concentration
Staph infection that is resistant to methicillin. How do we determine resistance?
MRSA; disk diffusion
Long structure used for propelling bacteria through medium. Common in Bacilli structures
Illness caused by a bacterium with a waxy outer layer
TB
This bacteria thrives on salt agar and produces a yellow ring around the colonies
Staph
After broth dilution and the MIC is determined, what happens next to determine the MBC
Culturing to find the Minimal Bactericidal Concentration (MBC)
Why would you not want to kill all E.Coli bacteria when you have a pathogenic E.Coli infection?
E. Coli is part of the normal flora. So, killing all E. Coli could make you sicker.