Measurement of Growth
Name 1 of 3 methods of measuring cell growth
Direct cell count, plate count, or turbidity based
How many phases of growth are there in a typical bacterial growth curve?
Four
Catabolism is when small molecules are assembled into larger ones, using energy. T/F?
False.
Name an example of a carbohydrate
Amylose, starch, glycogen, cellulose (fiber)
What classification do organisms that use light fall into?
Phototrophs
Which method of measuring growth measures VIABLE/LIVING cells?
Plate count/Colony forming units (CFU)
What is the name of the phase where there is an exponential increase in the number of living bacterial cells?
Log phase
Does glycolysis require oxygen?
No, anaerobic process
What is the name of the process where carbohydrates are synthesized from CO2 and H2A (electron donor) with light energy?
Photosynthesis
What classification of organism by energy and carbon source do humans fall into?
Chemoheterotrophs
If 2 cells are stuck together when doing a pour or spread plate in the plate count method, will they appear as 1 or 2 colonies?
1 colony.
What is the term used to describe the type of cell division that bacteria do?
Binary fission.
What is the name of the energy source cells utilize for energy? (SPELL OUT THE FULL NAME)
Adenosine Triphosphate
Proteins are made of what?
amino acids/peptide chains
Pathogenic microbes typically fall into which classification of energy & carbon usage?
Chemoheterotrophs
What is the name of the counting chamber used for direct cell count?
Hemocytometer
If you used an absorbance (turbidity based) method to construct a growth curve for Escherichia coli under a normal condition, how many phases of the 4 in the bacterial growth curve would you observe?
3
Where does the Krebs cycle take place
Mitochondrial matrix
What monosaccharide is used for glycolysis?
glucose
Why can bacteria and fungi be recovered from diverse environments?
Metabolic diversity
What is the name of the machine gauging cell count in the turbidity based method
Spectrophotometer.
What causes the plateau in the stationary phase of the bacterial growth curve?
Too many bacteria, limited resources/nutrients
The goal of the ETC (electron transport chain) is to restore what two things?
NAD+ and FAD
3 fatty acids and a glycerol make up what?
Name the primary producer of the deep sea hydrothermal vents
Chemoautotrophs