Tiddlywinks
ProtoPower
Virginia Tech...niques
What Am I?
Disease Diagnosis
100

Viruses, fungi, bacteria, and protozoa are all examples of __________-parasites.

What are micro-parasites?

100

__________ are unicellular eukaryotes.

What are protozoa?

100

This diagnostic technique involves looking at an organism's feces to determine the presence of a parasite. (Hint: giardia)

What is fecal examination?

100

Although I am rarely parasitic, I do enjoy indulging in a tasty treat now and then through my cytostome. I'd say I'm an overachiever, considering I have and macro- and micro- nucleus. As a young cyst I'd say I was pretty dormant, but I'm very active as a trophozoite and like to move around a lot, though my ex-left cause she didn't like that my whole body was covered in hair.

What is a ciliate and/or paramecium?

100

Jimmy brings in his sick pet fish. From what you can see there are small, raised white lesions all over its skin, fins, and glial tissue. Recalling your knowledge of parasites, you inform Jimmy that since his fish is the only host the parasite must have a ________ life cycle. 

What is direct life cycle? (P.S. you conclude that Bubbles has the parasite known as Ichthyophthirius multifilis)

200

Name at least one macro-parasite that we discussed in class. 

What is a flatworm/tick/leech/flea?

200

_______ ________ is the presence of two distinct nuclei (macronucleus and micronucleus) commonly in ciliates. 

What is nuclear dimorphism?

200

This diagnostic technique involves the use of the enzyme Taq polymerase and agarose gel electrophoresis. 

What is PCR and/or DNA sequencing?

200

My friends like to joke that I'm a gelatinous blob because I move around via my pseudopod. I mostly divide by binary fission and I like to think I'm very re-cyst-ent. Sometimes I flagellate and you'll find me soaking in warm waters.

What is an amoeba?

200

Your friend Hanna just returned from the tropics and is tested for Balantidium coli. She's not experiencing any symptoms, though the lab reports cysts being found in her stool. Hanna is confused as to how she's not sick even though she has the parasite. However, you know that cysts are the __________ stage in its life cycle and can cause infection, but not disease.

What is the infective stage?

300

Name at least one role parasites play in maintaining ecosystems.

What is remove the weak/remove top predators/disease regulates population size/ecosystem engineers/regulates food webs/parasites are food?

300

__________ is the temporary extension of the cytoplasm used for motility in some protozoa. (Hint: amoeba)

What is a pseudopod?

300

________ ________ is a diagnostic technique that utilizes protein migration through agarose gel and separates them based on their molecular weight. 

What is western blotting?

300

I only exhibit in the trophozoite stage and am usually nonpathogenic. I really like to move around and my favorite hobby is swimming.

What is a flagellate?

300

Fatima comes back from vacation in the Gulf of Mexico. While there for spring break, her and some friends decided to go cliff diving in a remote hot spring. After a few days, she begins to experience a high fever along with a headache, lethargy, disorientation, and eventually falls into a coma. Medical examiners later diagnose her with Naegleria fowleri, an __________ pathogen in humans that often leads to a fatal end. 

What is opportunistic?

400

___________ is the term used to describe a parasite that goes from a reservoir host to an intermediate mammalian host to a definitive human host. (Hint: an example of this would be rabies virus that travels from a bat to a raccoon and then to a human after being bit by the infected organism.) 

What is spillover?

400

Protozoa use this kind of nutrient acquisition.

What are heterotrophs/pinocytosis/phagocytosis?

400

________ ________ is a diagnostic technique that attaches a fluorophore-labeled secondary antibody that attaches to a primary antibody on the target protein to detect the presence of a parasite. (Hint: IFA)

What is immunofluorescence assay?

400

Dr. ________ and Dr. ________ are the main professors of this course. (Hint: I like to throw in a freebee once in a while ;)

Who is Dr. Ramirez and Dr. Gaji?

400

Charles drank some very poor quality water on his trip. Since returning from the Amazon, he has endured explosive, frothy, foul-smelling diarrhea. You are convinced he has contracted Giardia, which colonizes the upper portion of the small intestine. Because he is experiencing disease, the parasite is most likely in the __________ stage of the life cycle.

What is trophozoite/infectious?

500

__________ is a disease that is caused by prions and mostly affects deer. Its symptoms include a rough coat, altered mental status, emaciation, weakness, and loss of coordination. (Hint: CWD)

What is chronic wasting disease?
500

This style of asexual reproduction occurs longitudinally or transversely and is the most common method of asexual reproduction. 

What is binary fission?

500

__________ is the name of the field that studies and classifies single-cellular eukaryotic organisms and their life cycle. 

What is protozoology?

500

I'm usually the stage of life where everything begins. I can't do much, but I can establish an infection. I'm pretty popular and know how to make my way around. In fact, since I usually don't cause disease most people don't even know I'm there.

What is a cyst?

500

Ali has become infected with Giardia. As the infection develops, there is a 1-2 week period where there are no symptoms as the parasites grow. This period of time is known as the __________ period.

What is the incubation period?

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