Steps of PCR
Applications
Background of PCR
Materials in PCR
100

The process in which DNA samples are heated in order to seperate the strands.

What is Denaturation?

100

The international scientific research project with the goal of decoding the human DNA. 

What is the Human Genome Project?

100

A laboratory technique used to generate billions of copies of a sample of DNA through multiple cycles. 

What is Polymerase Chain Reaction/PCR?

100

A diluted sodium borate solution and specialized tubes. 

What are PCR Buffers and PCR Reaction Tubes?

200

The process in which short DNA molecules(primers) bind to regions of the DNA sample. 

Annealing

200

The study of genes and their heredity in offspring. 

What are Genetics?

200

This person was the inventor of the method of PCR. 

Kary Mullis

200

The target DNA that is intended to be copied

What is DNA Template?

300

The process where DNA Polymerase extends the 3' end of primers along the DNA sample. 

What is Extension?

300

The subset of genetics dealing with the manipulation and applications of Genetics. 

What is Genetic Engineering?

300

This was the setting, include time and place, that the process of PCR was invented. 

What is Cetus Corporation, 1983?


300

A pellet with the purpose of speeding up the PCR reactions and cycles. 

What is a PCR Reaction Pellet?

400

Repetitions of the three main steps of PCR about to create many copies. 

What are Cycles?

400

An application of PCR used to determine the male parent of an offspring. 

What is Paternity Testing?

400

The purpose of inventing the PCR lab method. 

What is to streamline the process of copying samples?

*Any variation of making the process of copying faster*

400

A common, heat resistant enzyme used to amplify DNA at heated temperature of 95 degrees celsius

What is Taq Polymerase?

500

A professionally made machine that streamlines the process of cycling. 

What is a Thermocycler/Thermal Cycler?


500

A process where geneticist use PCR to detect certain diseases based on DNA and ancestor health history. 

What is Hereditary Disease Detection?

500

The method of DNA copying before the invention of PCR. 

What is DNA cloning?

500

A solution of DNA molecules, commonly used in Agarose gel electrophoresis, as a reference to estimate band sizes of other samples. 

What is a DNA Ladder?

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