Blood collection basics
How long should a tourniquet remain on?
1 min
When is capillary puncture NOT appropriate?
When a light blue tube is needed
A blood smear from an EDTA specimen should be made?
Within 1 hour
What test needs to be protected from light?
Chain of custody is:
A special protocol used when collecting forensic specimens
Recommended needle angle for antecubital vs hand vein?
30° or less; 10° or less
Preferred adult finger for capillary stick?
Middle finger
Max time before separating serum/plasma?
2 hours
Department for Light pink, light blue, Red tops
Blood bank, coagulation, chemistry
Organization for specimen standards?
CLSI
Which test requires a 9:1 blood-to-anticoagulant ratio?
Prothrombin Time (light blue)
Safest infant heel puncture site?
Lateral plantar surface of the foot
Hemolysis falsely elevates what?
Potassium, magnesium, ammonia
Which of the following specimens needs to be chilled?
Ammonia
Citrate prevents clotting by binding ______________.
Calcium
The handling of a specimen before it is actually tested is part of the ________________ phase.
preexamination/preanalytical
Why collect EDTA first in capillary draws?
Minimize platelet clumping
Specimens least likely to need special handling?
CBC and glucose
EDTA, Na Citrate, Clot activator, Lithium hep: What are the tubes needed in the order of draw?
Light blue, SST/Gold/ Tiger Top, Light green, Lav
In phlebotomy, a fistula is a(n):
Permanent fusion of an artery and a vein
Prolonged tourniquet application may cause a change in blood composition primarily because of:
Hemoconcentration
Analyte higher in capillary blood?
Glucose
Wet alcohol before capillary puncture can cause?
Cause hemolysis of the specimen, Lead to errors in the testing process, Prevent round drops from forming
Which tests helps physicians differentiate between chronic obstructive pulmonary disease and congestive heart failure?
B-type natriuretic peptide (BNP)
The purpose of wiping away the first drop of blood during skin puncture is to:
Eliminate tissue fluid contamination