Safe and Sound
What is PPE?
Personal protective equipment
What pipettes do we use in class?
P10, P100, p1000
1000ul is ___ml
1
What can I find on MSDS?
Safety chemical info
Name the technique we use to be sterile in lab.
Aseptic
What is MSDS
Material Saftey data sheet
I need To pipette 4ul I’ll use..?
P10
234ml is ___ul
0.234ul
What is SOP?
Standard operating procedure
My tip touched the table what now?
Toss it and get another one
What colors are biohazard and glass bins?
Red and blue/white
I have 40ul in my pipette tip. What tube size is best?
1.5ml tube
900 in scientific notation
9x102
What 3 things go on a label?
Name/initials, date, substance
During an assessment there is no waste disposal and no safety glasses provided.
Ask assessor for waste disposal and safety glasses
T/F I can leave Etoh in the fume hood?
F. Ethanol belongs in the fire cabinet
What is the name of the tool used to pipette larger volumes?
Serological pipette
convert 2890ml to ul and give the scientific notation
2890000ul
2.89x106
T/F I can go back and write my batch record notes after the experiment is complete and fill in what I remember.
F
The tool used to transfer specimens from one agar plate to another?
Inoculation loop
Draw the safety symbol for oxidizing agent.
Instructor approval
At least 4
I’m diluting stock at 1:5 dilution factor what is the next dilution factor?
1:25
Name the document that is a combo of an SOP and record of your exp and usually has an MSDS attached.
Batch record
Draw a specimen plate and color in how you would streak it aseptically.
Instructor check