GalNAc Basics
What sugar is GalNAc derived from?
GALACTOSE
What does microwave spec measure?
Rotational transitions
What does CP-FTMW stand for?
Chirped-Pulse Fourier Transform Microwave spectroscopy
How many conformers were experimentally detected?
What are conformers?
Different 3D arrangements of a molecule
What functional group is added to galactose in GalNAc?
An Acetylated amino group (-NHCOCH3)
What type of molecules can be studied?
Molecules with a permanent dipole
What is the purpose of the chirped pulse?
to excite many rotational transitions at once
What type of hydrogen bond was strongest?
Carbonyl -OH hydrogen bond
What method was used to predict structures?
Density functional Theory (DTF)
What type of biomolecule is GalNAc?
An amino sugar
Why gas phase?
no interference/ allows for free rotation
What signal is emitted after excitation?
What interaction was weakest?
N-H to OH interaction
What determines conformer stability?
Intramolecular interactions (like H-bonding)
What does over expression of GalNAc indicate?
Cancer metastasis
Rotational energy levels
What converts FID into a spectrum?
Fourier transform
The major limitation is..
Fragmentation during laser ablation
What two main conformations were studied?
Syn and anti
what antigen is GalNAc part of?
Tn antigen
Why no signal for symmetric molecules?
No dipole
What is the purpose of the neon supersonic jet?
Cooling and isolating conformers
Why are results not fully biological?
Gas-Phase conditions are different from real environments
Which conformations were most stable
Syn Conformations