Amplification
What is a technique for making lots of identical of segment of DNA in living organisms?
DNA cloning
What is a dye used for DNA sequencing techniques ?
Fluorescent dye
What's use as a template for Northern Blotting?
mRNA
What is the important tool to recognize an snips DNA molecules at a particular nucleotide seq?
Restriction enzymes
When we say “DNA sequence” what is the sequence?
Seq = the order of nitrogenous base on DNA
What is an key enzyme that help synthesize cDNA from mRNA?
Reverse transcriptase
Name the enzymes that give hangover at cleavage
EcoRI, HindIII, PpuMI
How DNA sequences can be detected for Sanger methods?
By different color of fluorescent tag
Tell me steps of RT- PCR, by order
cDNA synthesis, PCR amplification, gel Electrophoresis
Heat tolerant enzyme employed in PCR?
Taq polymerase
How Dideoxy chain termination method differ from ordinary PCR?
Plus ddNTP
What is the technique that should be used to reveal gene expression in intact organisms?
in situ hybridization
What are the 3 processes in PCR
Denaturation, Annealing, Extension
Why we need ddNTP in Dideoxy chain termination method?
to terminate a growing DNA strand and give a sequence.
what kind of genetic material that be coated on glass slide in Microarray technique?
ssDNA