DNA Replication
What is the purpose of DNA replication?
Make a copy of the whole genome to make a new cell
What does PCR stand for
Polymerase Chain Reaction
what is bioinformatics
the study of biology using computers
where was taq polymerase found
in a bacteria in yellowstone hot springs
What phase of the cell cycle doe DNA replication happen?
S
Where does PCR occur
PCR tube/thermal cycler
What is sanger sequencing
Determining the sequence of DNA nucleotides
What is in a Sanger SEquencing tube
template DNA, primers, taq polymerase, NTPs, ddNTPs, buffer
Where does DNA replication take place
nucleus
What is the first step of DNA replication
Denaturation
What are some characteristics of ddNTPs
flourescent, chain terminating
What is in a PCR tube
Template dna, primers, NTPs, buffer, taq polymerase
What enzymes help with DNA replication
What are the temperatures of denaturation, annealing, and extension
95, 65, 72
what would happen if you didn't add normal NTPs to sanger sequencing
you would only get the 1st base of the dna sequence
What is annealing
primers form Hydrogen bonds with the template DNA
What direction does DNA polymerase BUILD DNA?
5-3
What is the difference between taq pol and dna pol
taq pol is heat resistant
If a new virus emerged, what would be the first step to studying it?
sequencing it
what are the 4 jobs that BLAST can do
DNA to DNA comparison, DNA to protein, Protein to DNA, Protein to protein comparison