Bacteria
These bacteria are spherical in shape.
Coccus
When performing electrophoresis on a gel, which electrode should the wells in the gel be closest to?
Negative (Black).
For a step in a lab protocol, you need to pipette 19 uL of a liquid into a tube. Which piece of equipment would do this most accurately?
the p20 (2 ul - 20 ul)
Which of the following items would not be found on an SDS (Safety Data Sheet) for a chemical? Substance Name, Chemical characteristics of the product, Cost of the Product, or Health hazards
Cost of the product
If the molar mass of Carbon is 12.01 and the molar mass of Oxygen is 16.00, what is the molar mass of CO2?
44.01
What is it about a bacterium that allows it to be gram stained?
The structure of its cell wall.
Which of the following would be a type of ancient biotechnology, practiced across history and ongoing to present day?
Fermentation, Spontaneous Generation, Cheese-making, Selective breeding, Making Yogurt.
Fermentation, Cheese-making, & Making Yogurt.
What is the function of a bioreactor?
Typically involves growing cells in a solution. by providing a controlled environment for biological reactions to take place in.
What is the correct sequence of substances listed below in the process of protein synthesis?
mRNA, amino acid, DNA, tRNA, protein, rRNA
DNA > mRNA > rRNA > tRNA > amino acid > protein.
What is the equivalent measurement for the amount shown below?
0.1 mL is equal to ____ uL
100 uL
During bacterial transformation, a plasmid (a circular piece of DNA) is inserted into a bacterium. This DNA provides the bacterium with the genes needed to become resistant to a specific antibiotic. How can this be useful in transforming bacteria with additional traits?
Any cell transformed with the plasmid will be able to grow on a medium containing the antibiotic.
Describe what a missense mutation is.
A type of point mutation where a single DNA nucleotide is substituted for another, resulting in a different amino acid being encoded at a specific position in a protein. This can alter the function of the protein.
How do you properly correct a mistake made in a lab write-up?
Put a single line through the mistake, so it is still readable and initial the changes made.
The regions within the DNA that do not code for the production of a protein are called:
Introns
A student creates a 1/2 dilution series (also known as a 1:2 or 50% dilution) of a chemical with the concentration of 1.5 mg/mL. How many times do they have to dilute the original solution in order to create a solution with a concentration of 188 µg/µL?
3
When labeling a Petri dish in a lab, on what part of the dish do you write your information?
The bottom of the dish.
The following substances are added to a PCR tube before it is placed in the thermocycler to undergo the process of PCR. There are two more ingredients needed. What are they?
Taq Polymerase, Forward Primer, DNTPs, and a Coenzyme.
Reverse Primer & Template DNA
What is the purpose for the following laboratory technique?
Flaming an inoculation loop before and after use
Sterilizes and prevents the contamination of a bacterial streak by unwanted bacteria.
In the description below, determine if it is describing DNA or RNA:
Acts as a temporary messenger or carrier of information. Composed of a single chain of nucleotides.
RNA
Which of the substances listed below would tend to make a solution more acidic? (Choose 2)
Citric Acid, Hydrochloric Acid, Sodium Hydroxide, Salt
Citric Acid & Hydrochloric Acid
What is the function of this DNA replication enzyme?
RNA Primase:
Adds RNA primers to the template DNA.
When doing a paternity test, what percent of the bands found in a child's DNA profile should be in common with each parent?
50% from Mom, 50% from Dad.
What is the purpose for the following laboratory technique?
Flaming the lip of a beaker/flask/tube
Creates a convection current that causes air to flow out of the container, reducing or eliminating contamination.
If a section of mRNA that is being translated has a total of 156 base pairs in it, how many amino acids will be in the polypeptide that is produced?
52
Chemical is labeled with a colored hazard label. What is the meaning of the color red?