Enter Title

Resolution and Detection

100

What is Pulsed Field Gel electrophoresis used for?

Large DNA strands

-Gives different currents to allow strand to move at different angles 

200

The horizontal comb is used for which of the following types of electrophoresis?

A. Agarose

B. Pulsed Field

C. Polyacrylamid

A

300

Is DNA + or - charged?

Negative

400

What is the purpose of Polyacrylamide gels?

Used for very small, single stranded DNA fragments

-Usesa catalyst rather than cooling

500

What are the 3 types of Electrophoresis gel systems?

Agarose, pulsed field, and polyacrylamide 

Resolution and Detection

100

What is capillary electrophoresis?

Polymer is inside capillary tube, rather than the solution

-Must use single stranded DNA 

-Flourcently labeled

-Needs to remain temp stable

200

The larger the DNA the ____ distance it will travel

The smaller the DNA the ____ distance it will travel

Larger: travels less

Smaller: travels farther

300

What is the purpose of the buffer in electrophoresis?

Carries the current and protects the sample

-Entire gel must be immersed in buffer

400

The higher the % of agarose indicates what?

Increased # of macromolecules

500

DNA migrates from the ___ end to the ___ end

Negative (cathode) to the positive (anode) end

Detection/Analysis

100

What is the Western blot the golden standard for?

HIV testing

200

The vertical comb is used for which of the following types of electrophoresis?

A. Agarose

B. Pulsed Field

C. Polyacrylamid

C

300

What are 3 common stains used to staining gel?

Bromide, silver stain, fast blast

400

What are the 2 things that the tracking dye does?

Gives a color so you can see the DNA move

Makes it heavier so it sits down in the well

500

What is the purpose of the DNA standard?

Gives us an idea where the sample will be

Analysis

100

What would the complementary strand be to this probe:

TAGCTTAGCCA

ATCGAATCGGT

200

What is the purpose of restrictive enzymes and where do they typically come from?

They cut specific/recgonized sites on DNA to characterize genes for clinical analysis a

Come from bacteria

300

The probe of the Southern Blot is complementary to what strand?

Targeted strand

400

What are the 4 steps in the Southern Blot testing process?

1) Cut with restriction enzymes

2) Seperate by electrophoresis

3) Denature and transfer to nitrocellulose blot

4) Hybridize labeled probe to complementary fragments

500

Is the probe in the Southern Blot single stranded or double stranded?

Single