Protein Concentration
The device used to measure protein concentration
What is a colorimeter?
This allows for the plasmid to replicate independently of the rest of the DNA
What is the origin of replication?
The reason that so much DNA is made from this reaction
The strands are doubled each cycle (exponential)
A site on an antigen that an antibody can bind to
What is an epitope?
Never store this in a lab freezer/fridge
What is food?
This experiment and an ELISA both show positive results with this
What is a color change?
Recombinant Plasmid
These are used in order to target synthesis to just the gene of interest
What is a pair of DNA primers?
This is responsible for the color change
What is the enzyme-substrate complex?
Preventing contamination of samples by micropipette is done by
Why do we change the tips constantly?
Finding the protein concentation of an unknown sample is done by this
What does finding protein concentration of known samples and plugging into a line equation show?
The three important genes in the plasmid we used to transform e. coli
Ampicillin resistance, glow gene, and arabinose activator
The reason for the Magnesium Ions
What is maintaining pH?
Goat anti-Rabbit IgG
What is an Antibody from a goat targeting a rabbit antigen
Preventing accidents is best done by
Possible error that occurs from skin oil
A cell that has been readied for transformation
What is a competent cell?
When the strands are broken up at 95 degree heat
What is the denaturation step?
Blocking of unbound sites on the wells
What is the purpose of washing with protein?
What is put on protective equipment?
A high protein concentration is indicated by
What does a high absorbance level show?
Cell membranes are weakened by this step just prior to plasmid intake
What is heat shock?
The type of polymerase used in PCR
What is a thermostable polymerase?
These wells should always change color to make sure the experiment is working
What is the positive control?
How do we prevent contamination of our petri dishes?
What is the clamshell method of opening?