microorganisms pt 1
microorganisms pt2
IHC pt 1
IHC pt 2
enzyme
molecular
100
the auramine-rhodamine technique will demonstrate
mycobaterium tuberculosis
100
Malaria is caused by
protozoan
100
an antigen is a substance that trigger the production of
immunoglobulins
100
in the indirect ihc method a,
a labeled secondary antibody raised against the FC portion of the primary antibody is used
100
Microscopic examination of sections of skeletal muscle stained with the a-naphthyl acetate esterase stain reveal a yellow precipitate on the sections. This could most likely have been prevented by:
washing sections more thoroughly after staining
100
What type of tissue is the best for genomic studies
frozen
fresh unfixed
200
For accurate diagnoses of bacterial diseases, fixed and unfixed tissue specimens should be submitted for
histologic and microbiologic studies
200
leishmaniasis is caused by a
protozoan
200
when an antibody labeled with a fluorochrome is reacted with tissue from a patient, the immunohistochemical technique is called
direct
200
T or F one standard standing protocol may be written to cover all specimens
False
200
What is the best long-term storage procedure for storing frozen muscle tissue used for enzyme histochemical stains?
-70°C
200
Blotting methods are done on
homogenized tissue
300
The carbol-fuchsin methods are specific for
mycobacteria
300
The PAS reaction will demonstrate fungi, because the cell wall contains
carbohydrates
300
which of the following chromogens is insoluble in alcohol
3,3'-diaminobenzidine tetrahydrochloride (DAB)
300
the following are functions of wash buffers in IHC except
mixing antibodies
linking reagents and labels
washing tissue sections
sterilizing slides
sterilizing slides
300
What enzyme technique demonstrates white blood cells of myeloid lineage?
chloroacetate esterase
300
The building blocks of DNA code is called
nucleotides
400
Proper use of the counterstain is important in the acid fast stains because
overstaining will result in masking of the organisms
400
In the Hotchkiss McManus modification of the pas technique, aldehydes are formed by
periodic acid
400
in the peroxidase anti peroxiase method of antigen detection, the pap complex is made in the same species as the
primary antibody
400
what condition is necessary for the secondary antibody in an indirect method
it must be raised against the IgG of the same species where the primary antibody has been raised
400
what is this staining procedure
α-naphthyl acetate esterase
400
T or F EDTA decalcified tissues cannot be used for ISH methods because the decalcification process will destroy the genetic material.
false
500
T or F: Viral organisms are easily demonstrated with special histochemical stains.
false
500
the developer in the warthin starry technique is
hydroquinone
500
which of the following link antibodies would most likely follow mouse monoclonal anti-kappa light chain primary antibody
rabbit anti-mouse
500
how is endogenous AP blocked
levamisole
500
what is this staining procedure
succinic dehydrogenase
500
T or F Ethanol and mercuric chloride are the preferred fixatives for ISH procedures
false
600
acid fast stains may be negative if the tissue was fixed in
carnoy solution
600
the stain shown here is dependent on the organisms
waxy capsule
600
T or F: negative reagent controls may be run by substituting for the primary antibody the diluent in which the antibody is prepared
true
600
Fixatives tend to mask antigenic sites and hamper immunohistochemical localization of antigens contain
aldehydes
600
If no enzyme activity is detected on histochemically stained sections of skeletal muscle, then the technologists should repeat the stain:
also staining a section of tissue known to contain the enzyme
700
scattered patchy blue areas are seen on the brown and hopps gram stain although the decolorization step was prolonged. This is most likely the result of
drying of the section during the procedure.
700
The reaction shown here depends on the presences of
aldehydes
700
T or F besides its use in tumor origin, prognosis and treatment, ihc can be used to aid in the demonstration of infections
True
700
After fixation for 24 to 48 hours in formalin, tissue for IHC should be stored in which of the following fluids to preserve antigenicity?
70% ethanol
700
Enzymes that act on substrates by adding water belong to the group known as
hydrolases
800
T or F: Helicobacter pylori is readily demonstrated by Romanowsky-type stain
true
800
the technique shown here is the
grocott
800
what are the major factors that require stringent control when performing enzyme induced epitope retrieval antigen unmasking
temperature and time
800
In preparing a renal tissue biopsy for immunofluoresence techniques, the critical anatomic structure to locate is:
glomerulus
800
The ATPase reaction involves both:
simultaneous coupling and metallic substitution
900
the light source for the technique shown is
halogen lamp
900
the section shown here has been
left in the impregnating solution too long
900
which of the following are common epitope retrieval methods
heat induced epitope retrieval and enzyme induced epitope retrieval
900
The tissue most often tested for diagnostics using fluorescent-labeled antibodies are
kidney and skin
900
The NADH diaphorase or reductase technique on muscle demonstrates abnormalities in
mitochondria, Z-band material, and sarcoplasmic reticulum
1000
the technique shown here is which of the following?
warthin starry
1000
while examining a GMS stained section microscopically it is noted that elastin, crenated red cells, and mucin have stained black, confusing the interpretation. This is most likely caused by
overexposure to hot silver nitrate.
1000
commercial monoclonal antibodies are produced by all of the following except:
several types of cells in the patients body
mice in lab
a single plasma cell and its clones
cell cultures
several types of cells in the patients body
1000
The orientation of a skin biopsy for immunofluorescent procedures must:
be perpendicular to the epidermal surface