Biochems
What select agent(s) are indole positive?
none, stupid
Name an assay that can be run on Quantstudio
Unknown panel, B. anthracis, Brucella
Who needs to be present to access the select agent inventory box in B133 freezer?
Name an extraction method for sputum for Y. pestis?
Qiagen DNA DSP blood mini kit
When opening the B133 refrigerator, what PPE must be worn?
Lab coat and safety glasses
Which organism(s) can utilize arginine?
Burkholderia mallei and pseudomallei
Which ABI can be used for Ebola PCR?
DX3
How many days do we have to destroy a select agent per DSAT and PHL policy?
7 days for DSAT
4 for PHL
Which extraction method is used for an unknown environmental sample?
What cleaning agent is used during work with Coxiella burnetii?
Microchem
Which antibiotic is read at 80 percent clearance?
Tetracycline
What mastermix is used for C. bot PCR?
Roche light cycler kit
How long do you have to disarm the SAIDS alarm before the police are alerted?
4 minutes
What is the role of cRNA in the Qiagen RNA extraction?
Increases RNA recovery and increases binding to RNA membrane
When do you have to wear a PAPR in B112 for C. bot testing?
During stool filtration
Describe the lysis of Y. pestis and Y. pseudotuberculosis at 25 and 35 degrees Celsius
Y. pestis lysis at both 25 and 35 while Y. pseudotuberculosis lysis only at 35
Which combination of BA targets indicate BA sterne?
BA1 and BA3
Name all select agents that we can keep in our inventory
Brucella and BA pasteur
how much wood can a woodchuck chuck if a woodchuck could chuck wood?
700 pounds or 6.573 pounds per day
What is the difference between a major spill and a minor spill?
Outside of a BSC is always a major spill. Inside of a BSC a major spill is > 1 square foot, and a minor spill is < 1 square foot.
Does F. tularensis require "x" and "v" factors?
nope.
What are the three different dyes used in the arbovirus PCR?
FAM, Texas Red, VIC
What is the DSAT form used to notify DSAT of a select agent identification?
Form 4, obviously
What volume of lysis buffer is used for a single sample during a MERS extractions?
200uL
What is the concentration of VHP that must be maintained for 90 minutes to achieve sterilization?
> 250 parts per million