General
How many public health laboratories are in Florida?
What is 3
True or False: HAI a Confirmatory Test
What is False
What type of ELISA do we perform?
What is IgM Antibody Capture (MAC)- ELISA
What does this result indicate (<1:10)?
What is a negative reading
HAI reports results on this day.
What is Fridays
Where is the Florida Public Health Laboratories Headquarters?
Where is Tallahassee, FL
What does HAI stand for?
What is Hemagglutination Inhibition Assay
What does ELISA stand for?
What is Enzyme-Linked Immunosorbent Assay
What does PRNT stand for?
What is Plaque Reduction Neutralization Test
True or False: HAI, ELISA, and PRNT results are on the confirmatory report.
What is False
Which lab is the only lab to test sentinel chickens in Florida?
What is the Tampa lab
True or False- Best Practice: Hemolyzed specimens can affect assay results.
What is True
What specimens go to ELISA for confirmation?
What are Presumptive Positives and Reactives
How long does the ELISA/PRNT confirmation assays take?
What is 2-4 weeks
Which birds are NOT shown on the confirmed Positive Report, even if they are positive?
What are new birds
Who is the current Tampa Virology Administrator?
Who is Lea Heberlein, DrPH
How many specimens can the HAI team test during peak season?
What is more than 1500 per week
What is an advantage of IgM ELISA? Choose from these options (Cost, Cross-Reactivity, Increased Specificity, or Labor Intensive)
What is Increased Specificity
What are the two advantages of using the PRNT method?
What is high specificity and the use of chimeric viruses
What does this box represent (image)?
What is Lab ID (slide 42)
Why are chickens an ideal sentinel animal?
What is a measurable immune response
What is the minimum volume of serum required to be sent for testing (in microliters) in HAI?
What is 500 microliters
What chemical is used to stop the substrate reaction?
What is sulfuric acid
Which specimens go to PRNT for confirmation? List 2.
What are Positives (Not Confirmed), Negatives, Equivocal, & Inconclusive
What is the name of this sheet (image)?
What is the Sentinel Flock Arthropod-Borne Virus Surveillance Summary (Surveillance Summary) slide 46