Chapter 33
The Limulus Amoebocyte Lysate replaces which other test?
A) Mouse Lethality Test
B) Rabbit Pyrogen Test
C) Draize Eye Irritation Test
D) Rabbit Vaginal Irritation Test
B) Rabbit Pyrogen Test
Example of replacement!
Tests for endotoxin
Which correctly explains the "Bruce effect" in mice?
A) Early induction of first estrus after exposure to sexually mature male urine
B) Pregnancy block – females terminate their pregnancy following exposure to unknown male
C) Synchronization of estrus after exposure to male pheromones
D) Renewed sexual interest when a new female is introduced
B) Pregnancy block – females terminate their pregnancy following exposure to unknown male
A) Early induction of first estrus after exposure to sexually mature male urine - Vandenberg
C) Synchronization of estrus after exposure to male pheromones - Whitten
D) Renewed sexual interest when a new female is introduced - Coolidge
Which country is not apart of IACLAM?
A) Japan
B) Europe
C) Korea
D) Australia
D) Australia
Only one ACLAM, no AACLAM
The "C" strain abbreviation stands for which strain?
A) C57BL
B) BALB/c
C) CBA
D) C3H
B) BALB/c
What strain would have the coat color from this genetic combination?
C/C a/a B/b D/d
A) C3H
B) DBA
C) BALB/c
D) C57BL6
D) C57BL6 - black
A) C3H- Agouti C/C A/a B/b D/d
B) DBA- Dilute brown C/C a/a b/b d/d
C) BALB/c- albino c/c plus anything else
Which animal has an orbital plexus?
A) Chinchilla
B) Gerbil
C) Hamster
D) Guinea Pig
D) Guinea Pig
FRROG - Ferret, rat, rabbit, opossum, GP
Best light cycle (L:D) to promote reproduction in Mesocricetus auratus?
A) 8:16
B) 14:10
C) 10:14
D) 16:8
B) 14:10
Also zebrafish
Mustela putorius furo - 16L:8D
Who represents LAM in the AVMA house of delegates?
A) ACLAM
B) ASLAP
C) AALAS
D) AAALAC
B) ASLAP
•Foster educational activities in LAM and advance LAM through member development and representation
•Veterinary Student Liaison Committee (VSLC) – faculty representative at vet schools
Cross two inbreds, then backcross F1 hybrid to one of the parental strains (recipient), then subsequent inbreeding (bro x sis) for 20+ generations?
A) Conplastic
B) Consomic
C) Recombinant congenic
D) Advanced intercross
C) Recombinant congenic
Backcrossing = congenic
CcS (RC strain between BALB/c recipient and STS donor)
What genetic mutation results in a model for x-linked agammaglobulinemia?
A) Btkxid
B) Ptpn6me
C) Foxn1nu
D) Rag1tm1Mom
A) Btkxid
Survival cardiac puncture is not permited with which species?
A) Marmota monax
B) Monodelphis domestica
C) Didelphis marsupialis
D) Boa constrictor
C) Didelphis marsupialis - common opossum
B) Monodelphis domestica - lab opossum is allowed
Which statement on sexual dimorphism in mice is correct?
A) Females secret 4x as much allergens in their urine
B) Male kidneys tend to be larger than female kidneys
C) Females will have cuboidal epithelium lining the parietal layer of Bowman’s capsule
D) Females will have an increased anogenital distance
B) Male kidneys tend to be larger than female kidneys
Which organization was created during the Food Security Act of 1985?
A) LAMA
B) ILAR
C) AWIC
D) ICLAS
C) AWIC
Animal Welfare Information Center
USDA’s National Agricultural Library
Also implemented IACUC, exercise for dogs, primate enrichment, and alternatives for painful/distressful procedures
B6-mtCAS is an example of?
A) Coisogenic
B) Congenic
C) Conplastic
D) Consomic
C) Conplastic
Male nuclear genome of one inbred strain backcrossed to female cytoPLASmic genome of a different inbred strain for 10 or more generations. Look for “mt”. Only case where the male is listed first!
What gene is responsible for the Piebald syndrome?
A) Cdh23ahl
B) Ednrb5
C) Oca2p
D) Prkdc
B) Ednrb5
Associated with aganglionic megacolon (just like piebald horses)
According to a recent journal article, what novel method can be used for terminal blood collection in Xenopus Laevis?
A) Decapitation
B) Enucleation
C) Mid-tibiofibular amputation
D) Intercostal retraction
C) Mid-tibiofibular amputation
"Mid-Tibiofibular Amputation as a Method of Terminal Blood Collection in Xenopus Laevis"
The leg amputation method produced significantly higher blood volumes in shorter times and showed no significant differences in clinical pathology parameters as compared with cardiocentesis.
When is peak antibody absorption in neonatal rats?
A) 24 hours
B) 7 days
C) 14 days
D) 21 days
C) 14 days
"This transfer occurs at low rates shortly after birth, reaches maximal rates at day 14, and ceases by the 21 days, when gut closure is said to be complete”
What is not a requirement for ACLAM training programs?
A) Trainees must complete at least 24 months of formal training
B) At least 200 hours of direct faculty involvement
C) Species exposure will vary among training programs but at minimum 100 hours of contact with primary species.
D) At least 2000 hours of supervised experience in the practice of laboratory animal medicine
C) Species exposure will vary among training programs but at minimum 100 hours of contact with primary species.
•Species exposure will vary among training programs and among trainees within a program; there is no minimum requirement for hours of contact by species.
What category of inbred mouse strain is represented by the following nomenclature
B6.129P2-Il10tm1Cgn/J?
A) Coisogenic Strain
B) Conplastic Strain
C) Consomic Strain
D) Congenic Strain
E) F2 Hybrid
Answer: D) Congenic Strain
Reference: 1) MGI “Guidelines for Nomenclature of Mouse and Rat Strains” International Committee on Standardized Genetic Nomenclature for Mice, revised: October 2011, http://www.informatics.jax.org/mgihome/nomen/strains.shtml
2) Laboratory Animal Medicine, 3rd edition, 2015, p. 52.
Domain 3-Primary Species
Coisogenic: Strain - gene
The Moth Eaten mouse strain is used for what kind of research?
A) Recombination
B) Apoptosis studies
C) Muscular Dystrophy
D) Chediak Higashi
B) Apoptosis studies
The Corriedale sheep is a model for which disease?
A) Dubin-John Syndrome
B) Gilbert's Syndrome
C) Crigler-Najjar Syndrome
D) Guillain-Barre Syndrome
A) Dubin-John Syndrome
B) Gilbert's Syndrome - Southdown
C) Crigler-Najjar Syndrome - Gunn Rat
D) Guillain-Barre Syndrome - Rabbit ileal loop ligation
What pH has been shown to cause extensive enamel and dental erosion in rats?
A) 4
B) 3.5
C) 2.5
D) 2
D) 2
pH 2.5-3 good for P aeruginosa
How long do you have to correct deficiencies with a deferred accreditation by AAALACi?
A) By the next annual report
B) 12 months
C) 2 months
D) up to 24 months
C) 2 months
NEW applications
Accreditation with conditions -> By the next annual report (still accredited)
Award Provisional Status -> Up to 24 months (NOT accredited)
RENEWALS
Conditional Accreditation -> by the next annual report (still accredited)
Deferred -> 2 months (still accredited)
Probation -> 12 months (still accredited)
Which of the following breeding systems is perpetuated by non-brother-sister matings?
A) C57BL/6Jei-tth
B) B6.AKR-H2k
C) SHR-ChrYBN
D) Pri:B6,D2-G8
E) CcS1
Answer: d. Pri:B6,D2-G8
References: 1) Laboratory Animal Medicine, 3rd Edition, 2015, p. 46 2) The Mouse in Biomedical Research, p. 71.
Domain 4
A: coisogenic
B. Congenic
C: Consomic
D: Advanced intercross line (Start with F2 generation, intercross mice but avoid sibling mating)
E: recombinant congenic
The gene responsible for the immunodeficiency in Beige mice is found on which chromosome?
A) Chromosome 2
B) Chromosome 10
C) Chromosome 11
D) Chromosome 13
D) Chromosome 13
Lyst locus, Chediak Higashi, NK cell deficiency
A) Chromosome 2 - Agouti, Rag1/2
B) Chromosome 10 - 10 – Kit/, Cdh23ahl
C) Chromosome 11 - 11 – Foxn1nu