Transcription
RNA modification
Translation
Enzymes
Random
100
Which Direction does RNA polymerase build?
5' -> 3'
100
True or False
All RNAs must go through intron removal
False
Bacteria do not have introns in their RNA to remove
100
True or False
Ribosomes read 5' -> 3'
True
100
What does a RNA holoenzyme consist of?
A core RNA polymerase and a sigma factor
100
True or False:
immediate transcription-translation can occur in both prokaryotes and eukaryotes
False. Eukaryotes have a nucleus
200
Name the three important numbered sequences in a promoter region
-35 sequence, -10 sequence, and +1 sequence.
200
Splicing
Introns in group 1 and 2 can _________
Introns in group 3 require a __________
Self-splice
Spliceosome
200
True or False
The tRNA that matches a STOP codon, adds methiodide to end the polypeptide chain
FALSE
There are no tRNAs that match stop codon sequences.
200
What does the torpedo model require to remove RNA pol II from the DNA?
an exonuclease
200
This protein complex facilitates interactions with RNA pol II and regulatory transcription factors
The mediator
300
Double Stranded bacterial DNA is separated at the A-T rich -10 region to form the ____________
Open complex
300
This uncommon method of RNA modification occurs when a base sequence of an RNA molecule is changed
RNA editing
300
What does this codon code for? 5'-CAU-3'
HIS
(histidine)
300
What enzyme breaks the phosphodiester bond between nucleotides at the END of a strand of tRNA?
Exonuclease
300
What did Beadle and Tatum use to break the protein coding genes in DNA?
X-ray
400
If a rut site is mutated and loses it's function, what will happen to the mRNA?
The ρ protein will not bind, and transcription will not be terminated
400
This is what it is called when a 7-methylguanosine is added to the 5' end of the pre-mRNA
5' capping
400
The amino acid is attached to the tRNA at what structure?
The Acceptor Stem
400
What are the subunits of a bacterial ribosome?
30S+50S
400
What is the difference between a protein and a polypeptide?
A polypeptide is a string of linear amino acids, whereas a protein is a string of covalently bonded amino acids that are FOLDED into a specific shape.
500
What is the order that transcriptional factors bind in?
1. (TFIIA)TFIID
2. TFIIB
3. TFIIF
4. TFIIE
5. TFIIH
500
What method of RNA modification helps
1 facilitate mRNA export from nucleus
2 Stabilize mRNA in cytosol
3 translation of the mRNA
3' polyadenylation
500
What is the order of the sites that the mRNA goes through in ribosomes?
1. A site (aminoacyl site)
2. P site (peptidyl site)
3. E site (exit site)
500
How many RNA polymerases do Eukaryotes have?
Which one transcribes mRNA?
3
RNA pol II
500
Given the anticodon 5'-AAG-3', what are all of the codons that can be used to get the same amino acid
Codons:
5'-CUU-3', 5'-CUC-3',5'-CUA-3',5'-CUG-3'
All code for Leu
(leucine)