Fragment ratios
what fragments would you get from a molecule with a 57:15 ratio
parent molecule: CH3CH2C(=O)CH3+
Fragment 1 (57):CH3CH2C=O (+)
Fragment 2 (15): CH3+
Ionization
Conversation of molecules to ions
mass spectra is used in the field for: water testing, pesticide screening, and CO2 monitoring
Environmental
Ions are attracted to the negative or positive side of the electromagnetic field allowing the ions to be separated
Deflection
The name of the process that gives a molecule or an element a charge?
✨ionization✨
If fragment 1 is CH3CH2+ what is fragment 2?
CH3C(=O)+ (bonus: ratio is 29:43)
Fragmentation
The process of breaking a molecule into a cation and a radical
Mass spectra in this field is used for: trace evidence identification, arson detection, and identifying explosive residuals
Forensic analysis
electrons are knocked off the sample creating mostly positive cations
Ionization
The breaking of a molecule usually from electron bombardment
Fragmentation
If fragment 1 is CH3+ then what is fragment 2?
CH3C(=O)CH2+ (bonus: ratio is 15:57)
Base Peak
The tallest peak detected in a mass spectrum (bonus:usually has an intensity of 100)
Mass spectra in this field is used for: Drug ADME studies, metabolite screening, and preclinical developments
Pharmaceutical analysis
The sorted ions collide with a metal plate producing a signal sent to a computer
Detection
Simple math skills used to read mass spectra’s and calculate charges from ionization
Subtraction and addition
What do the fragments of CH3CH2C(=O)CH3+ with a fragment ratio of 43:29?
Fragment 1: CH3C(=O)+
Fragment 2: CH3CH2+
Molecular ion
A cation with an unpaired electron and +1 charge
Mass spectra in this field is used for: clinical drug testing, disease testing, and drug therapy monitoring
Clinical
Ions move through charged plates creating a narrow beam of high speed particles with equal kinetic energy
Acceleration
The process used in ionization to knock off electrons
Electron bombarding
What is the total m/z of the molecule CH3CH2C(=O)CH3+
72 m/z
M Peak
The parent molecular ion peak (has a m/z ratio to the molecular weight of the original compoun)
mass spectra in this field is used for: characterization of proteins, and sequencing of peptides
Protein studies or Proteomics
What side do heavier ions deflect to the positive side or negative side?
Heavier ions go to the positive side and lighter go to negative
Units used for fragment ratios after ionization
m/z