carbohydrates
lipids
proteins
water
food test
100

What is the H:O ratio in carbohydrates?

2:1

100

What is the H:O ratio in lipids? 

larger than 2:1

100

What elements are found in proteins? 

carbon (C), hydrogen (H), oxygen (O), nitrogen (N) and sometimes sulfur (S)

100

How would you test for the presence of proteins in food? 

Using a protein test strip
200
How does a disaccharide form from two monosaccharides?  And how do we get monosaccharides from a polysaccharide? 

Condensation reaction ;

Hydrolysis reaction;

200

How to make a triglyceride molecule? 

A triglyceride is formed from one glycerol and three fatty acids by condensation reaction.

200

Name some food that is rich in proteins 

meat, fish, eggs, beans, milk, dairy products (e.g. cheese)

200
How would you test for the presence of starch in food? What is the positive result? 
Iodine test.

If starch is present, the iodine solution turns from brown to blue-black.

300
Name some food that is rich in:

i) starch 

ii) fructose

iii) lactose

i) rice, wheat, potatoes, taros 

ii) fruits, honey

iii) milk, dairy products such as cheese and yogurt

300

Name some food that is rich in lipids. 

Fatty meat, seeds, nuts, milk, dairy products (e.g. cheese)

300

What is the basic building block of proteins? Describe its structure.

Amino acid;

Each amino acid has an amino group, a carboxyl group and a side chain. Different amino acids have different side chains. 

300

How would you test for the presence of reducing sugars in food? What is the positive result? Can you use this test to detect sucrose? 

- Add the food sample to Benedict's solution and heat in water bath. 

- If reducing sugar is present, a brick-red precipitate will form.

- Sucrose is not a reducing sugar so no.


400

Name three polysaccharides and state their functions.

Starch - major energy source in our diet

Glycogen - energy storage in animals

Cellulose - a component of plant cell walls

400

Name 4 functions of lipids

- as energy reserves

- as a shock absorber

- as a heat insulator

- produce hormones

- a component of cell membrane

- involved in the absorption, transport and storage of lipid-soluble vitamins

400

State 3 functions of proteins. 

- for growth and repair

- to produce enzymes, antibodies, haemoglobin and some hormones

- as a source of energy when carbohydrate and lipid storage are used up in body

400

State 4 significance of water in living organisms. 

- act as a reactant in chemical reactions (e.g. hydrolysis)

- provide a medium for chemical reactions to take place

- a good solvent and a good transport medium

- act as a cooling agent 

- provide lubrication (e.g. in joints)

- provide buoyancy for aquatic organisms

400

Describe how you would carry out a test to detect the presence of lipids in food. 

- Put a drop of food sample onto a piece of filter paper and let it dry.

- A translucent spot will remain on the filter paper if lipids are present.

- If this translucent spot is formed by lipids, it will disappear if we put it into an organic solvent (e.g. ethanol). This is because lipids are insoluble in water but soluble in organic solvents. 

500

What determines the final shape of a protein? Use an example to illustrate why its unique shape is important to its function. 

The sequence of amino acids determines the final shape of a protein.

Its shape is important. For example, enzymes have unique active sites which fit with specific substrates. Changes in the shapes of the active sites (i.e. denaturation) causes enzymes to lose their functions. 

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