HEME
HEME
HEME
General
100

How many d electrons Fe has in TPPFe-Superoxo

5

100

What is the bond order of molecular oxygen, TPPFe-superoxo and TPPFe-peroxo

2, 1.5 and 1

100

The porphyrin ring in heme is composed of how many pyrrole rings?

4

100

Which form of iron binds oxygen in heme?

Fe2+

200

Which type of heme protein typically gives an axial EPR signal at g ≈ 6 and g ≈ 2?

High-spin ferric myoglobin

200

Which oxidation state of iron in heme is EPR-active?

Fe³⁺ (high-spin)

200

Temperature in EPR measurements of heme proteins is typically kept low to:

Increase signal-to-noise ratio

200

Which prestigious award did Dr. Wijeratne receive in 2024 to fund his investigations into nitrogen oxide–heme chemistry?

NIH R35 MIRA Outstanding Investigator Award

300

 EPR observations suggest a substrate-bound (low-spin) state in P450 enzymes. What are the g values?

g = 2.4, 2.2, 1.9

300

What does a rhombic EPR spectrum indicate about a ferric heme center?

Anisotropic (low symmetry) environment

300

Which test detects porphyrin accumulation in urine?

Watson-Schwartz test

300

The lab uses cryogenic setups during experiments primarily to:


Preserve reactive intermediates for spectroscopic analysis

400

Which enzyme catalyzes the breakdown of heme to biliverdin?

Heme oxygenase

400

Which gas binds to heme with higher affinity than oxygen?

Carbon monoxide

400

In cytochromes, what is the primary role of heme?

Electron transfer

400

What key mechanistic process is being probed by the lab’s synthetic model systems involving heme and O₂ or NOₓ?

Dioxygen activation and nitrogen oxide interconversion

500

Which enzyme catalyzes the rate-limiting step of heme biosynthesis?

 ALA synthase (ALAS)

500

Heme is an essential component of which electron transport chain protein?

Cytochrome c

500

Which organ is primarily involved in regulating heme synthesis?

Liver

500

What is the primary research focus of the Wijeratne Lab?


Bioinspired model complexes of heme enzymes activating O₂ and NOₓ

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