Microbiology Basics
Prokaryotes vs. Eukaryotes
Viruses and Infections
Bacterial Structures
Staining and Microscopy
Growth, Media, and Metabolism
1

These are living organisms too small to be seen without magnification.

What are microorganisms?

1

This type of cell lacks a nucleus and membrane-bound organelles.

What is a prokaryotic cell?

1

Viruses are best described as __________ (cellular or acellular?).

What is acellular?

1

This structure provides shape and protection and contains peptidoglycan.

What is the cell wall?

1

 The Gram stain is classified as this type of stain.

What is a differential stain?

1

The first of the Five I’s of microbiology

What is inoculation?

2

Microbes that break down dead organic matter and are usually nonpathogenic.

What are decomposers (saprobes)?

2

The region where prokaryotic DNA is found.

What is the nucleoid?

2

The protein coat surrounding viral genetic material.

What is a capsid?

2

Appendages primarily used for bacterial attachment.

What are fimbriae?

2

Gram-positive bacteria stain this color due to thick peptidoglycan.

What is purple?

2

This type of growth medium allows some microbes to grow while inhibiting others using chemicals such as bile salts or antibiotics.

⭐️ What is selective media?

3

Microorganisms that cause disease in a host.

What are pathogens?

3

These ribosomes are found in bacteria and are targeted by antibiotics.

What are 70S ribosomes?

3

 Correct order: adsorption → ___ → synthesis → assembly → release

What is penetration/uncoating?

3

The appendage responsible for bacterial motility.

What is a flagellum?

3

This property of microscopes depends on the wavelength of illumination and determines how well two close objects can be distinguished.

⭐️ What is resolving power (resolution)?

3

This solidifying agent is not degraded by most microbes.

What is agar?

4

Energy-producing microbes that metabolize sugars anaerobically

What are fermenters?

4

Name one structure present in eukaryotic cells but absent in prokaryotes.

What is a nucleus? (or mitochondria, ER, lysosomes)

4

This viral cycle integrates viral DNA into the host genome without killing the cell immediately.

What is lysogeny?

4

This bacterial structure enhances survival in extreme conditions and is especially common in Bacillus and Clostridium species

⭐️ What is an endospore?

4

This microscope uses electrons and has a resolution limit of ~0.2 nm.

What is an electron microscope?

4

 Media with an exact known chemical composition.

What is chemically defined media?

5

This process involves separating individual bacterial cells to obtain a pure culture.

⭐️ What is isolation?

5

This theory explains how mitochondria and chloroplasts originated from bacteria.

What is the endosymbiotic theory?

5

In this type of viral infection, the virus remains dormant in the host cell and may reactivate later.

⭐️ What is a latent infection?

5

 This outer membrane component of Gram-negative bacteria contributes to pathogenicity.

What is lipopolysaccharide (LPS)?

5

The staining technique used to diagnose tuberculosis.

 What is an acid-fast stain?

5

The universal energy currency of the cell.

What is ATP?

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