Shake Flask Operations
What is the amount of days of cultivation for Passage 3?
3
True/False. When additional material is needed for 50L operation, a note is required in the batch record.
True.
What is the item number for the Levitronix pump chamber?
Item # 8120
True/False. The inoculation target volume is calculated by the inoculation recipe in MPCS.
False. The inoculation target volume is calculated by operators.
True/False. The correct shorthand code for a time error is "TE."
False. There is no shorthand code for a time error. "TE" should be used for a transcription error.
What is the volume of media required to warm for Passage 2?
50.0mL +/- 0.5mL
The following scenario is correct: NOVA pH: 7.01 Controlling probe: 7.03, Non-controlling probe: 7.08 Correct both probes to the NOVA pH.
No it is not correct. No correction to the controlling probe. Correct the non-controlling probe to match the controlling probe.
Find the error in the following statement: Person A recorded the NOVA metabolites in the batch record. They record "N/A" for the Lactate. Person B verifies. Both operators proceed to the next step.
Operators should have contacted MS&T and collected a non-routine sample. A footnote should have been made in the corresponding step.
What is the governing TD for Tsuru control strategy?
TD21865
What is the SOP governing Good Documentation Practices?
SOP-0028
What is the SOP governing Shake Flask operations?
SOP-2262
What is the document number of the 50L bioreactor operation?
BR-1850
True/False. The operators taking the daily sample must be the same operator to record the date/time.
False. The operator taking the daily sample is not the operator recording the date/time.
True/False. The time window to submit a supernatant sample to sample coordination is 4 hours.
True.
What is wrong with the following correction? Person A records 1.58 but the Batch Record states the number of decimals is one. Person A then crosses out the number and corrects it to 1.6 and makes a footnote stating "RE."
"RE" is not a valid shorthand code. The correct footnote should state "rounding error."
True/False. The shake flask cannot be moved into the incubator while Vi-Cell results are being obtained.
False. The shake flask can be moved into the incubator while the Vi-Cell results are being obtained.
True/False. Potassium (K+) and Calcium (Ca++) are required metabolites for daily samples.
False. Potassium (K+) and Calcium (Ca++) are not required metabolites for daily samples.
What value are the DO probes spanned to prior to inoculation?
760.00 mmHg
What value must the Glucose fall under to require a Glucose bolus?
2.00 g/L
True/False. Both date formats are correct: 22Mar2026 and 22March2026
True.
What is the minimum volume of air that needs to be sampled before stopping environmental monitoring?
1m3
What is the value in the following PO2 calculation:
149.3 mmHg ÷ (x) mmHg = 93.9%
1.59
What is the media equilibration duration?
12 - 36 hours
What is the allowable response period for pH?
30 minutes
True/False. The use of slashes or hyphens (ex: Ø) are not permitted.
False. The use of slashes or hyphens are permitted.