Flourometry
Why do we freeze samples for Fluorometry?
To lyse the cells and release pigments.
What are the two most abundant cyanobacteria genuses found on Cape Cod?
Microcysis and Dolichospermum
How long after the net tow can the sample go on the ZAPPR?
2 hours
How many times do you rinse the sample bottles?
3 Times!
Finish the sentence: When in doubt _____ ______
Stay Out
Once the thawing process begins the samples need to be analyzed within ___ minutes to avoid pigment degredation.
20
Why does APCC use microscopy in addition to fluorometry?
Microscopy determines the dominance which guides toxin testing.
Why is there a clear tube at the bottom of the ZAPPR?
To attract zooplankton
Why do we use the integrated tube?
Integrated tube is used to capture the whole water column.
What are two ways that cyanobacteria blooms can cause harm?
Producing toxins and using up disolved oxygen.
What are some things you should always do before a fluorometry reading?
- invert vial
- take temperature
- wipe off cuvette
Why do we not want bubbles in our slides?
Sedgwick rafters hold exactly 1mL, if there are bubbles an incorrect volume of water is in the slide. This will lead to inconsistencies in our dataset.
How many hours are samples supposed to freeze before you run flourometry?
What are the three photos we take in the field?
One of the sho
They are responsible for the great oxygenation event!
Why do we use phycocyanin as an indicator for cyano biomass rather than chlorophyll a?
Because all alage produce chlorophyll a but only blue green alage produce phycocyanin.
Count this slide!
4 MC
5 DS
1 AZ
If there is a confirmed significant cyanobacteria scum and the average BFC value was 43. What risk tier is it?
-Acceptable
-Potential for Concern
-Use Restriction Warranted
Acceptable
What do you do when you see pollen on the shoreline?
You take the scum sample! You do not know if the scum is pollen or not until you look it under the microscope!
How can you predict cyanobacteria blooms?
You can not
What color is the phycocyanin pigment?
blue!
What do you do if you can't identify a cyano in your slide?
Get a second opinion and try to agree to the family or order level making sure to note "cf". Upload lots of pictures!!
Massachusetts Department Public Health recommends public health advisory for an exceedance of microcystin at ____ ug/L
8
Why don't we want the bottle to overflow when doing a net tow?
The net captures a specific number of biomass based on the actual concentration of alage on the surface, if the bottle overflows we are not capturing an accurate sample.
Provide answers to the following:
1. What is one method for removing nutrients from waterways?
2. What is one method for decreasing nutrient inflow?
3. Why is nutrient removal a "band-aid"?
Various correct answers.