B
What demographic information is acceptable for labeling patient samples?
First name, last name, MRN, DOB, BBID
Incubation time of 30 minutes
Albumin
Incubation time of saline
60 mins
Which of the following is a valid source of passively acquired antibodies detected in a patient’s specimen?
Anti-D detectable in obstetric patients who received Rh immune globulin
Maternal antibodies that cross the placenta from mother’s plasma into the circulation of the fetus
Unexpected anti-A or anti-B due to transfusion of ABO incompatible plasma products
All of the above
Which fluid should be used to dilute RBCs when being transfused?
0.9% normal saline
What best describes the outcome of hemolyzed samples that arrive for pretransfusion testing?
Redrawn
Dosage is described as:
The reactivity strength of an antigen based on its homozygosity or heterozygosity
Once the golden rule of 3 is met what other test will further confirm the identity of the antibody recovered?
Antigen type the patient for the corresponding antibody
Extra point - What should this result be and why?
What reagent gives us an advantage by providing the lowest incubation time? What is the incubation time?
LISS - 15 mins
The golden rule for antibody identification follows a rule of 3 meaning what?
Having 3 antigen positives and 3 antigen negatives in a panel
Which of the following is the most significant serologic test when performing pretransfusion testing?
ABO
Enzyme examples
Ficin,Papain,Trypsin
Red blood cells are to be given to a patient typing B POS. Which of the following ABO types will be most compatible with the patient?
B
Red blood cells are to be given to a patient typing O POS. Which of the following ABO types will be most compatible with the patient?
O
Fresh Frozen Plasma is ordered for a patient typing B NEG. Which of the following ABO types will be most compatible with the patient?
B
A false-negative antibody screen could be due to:
A weak antibody reacting with selected homozygous cells only, showing dosage
An antibody to a low-incidence antigen not found on screening cells
Patient serum not added to test tubes
All the above
All the above
Which of the following best describes a Type and Screen request? A specimen and request form are submitted to the blood bank and:
Perform ABO/Rh typing and an antibody screen. Store the sample until blood products are requested.
While performing pretransfusion testing, the antibody screen was found to be positive. What is the BEST step to take next to provide blood products to the patient?
Perform an antibody identification panel
How do enzymes work?
Proteolytic enzymes cleave proteins from the surface of RBCs, destroying some antigens while enhancing others.
If a panel has a positive auto control what test is performed to test for RBCs coated in vivo?
DAT
A pretransfusion test that uses red cells from the donor unit and plasma from the patient is known as a:
A Crossmatch test
If pretransfusion testing indicates the patient has no clinically significant antibodies and no history of previous antibodies, which serologic crossmatch method should be used when RBC products are requested for transfusion?
Immediate spin crossmatch
When antibodies are present in the current patient specimen or in the patient’s blood bank history, which crossmatch method should be used?
Antiglobulin crossmatch with units negative for corresponding antibody
An emergency room physician calls the blood bank for emergent release red blood cells. The male patient has a birthdate of 3/17/1996. What is the BEST blood type to release prior to any compatibility testing on the sample?
O Pos
How do you perform an antibody identification on a panel?
Homozygous antigens are ruled out when positive on a negative reaction for the cell tested.