Microscopes
use combination lenses and mirror to illuminate thin specimens
compound light microscopes and bright field microscopes
one of the failures still of fluorescent microscopy and the other microscopes
still could not see or identify specimens as small as a virus or DNA molecure
SEM/ TEM
maximum magnification was around 1000 times, due to limited resolution
compound light microscopes or bright field micropscopes
these microscope solved the issue of viewing tiny specimens such as virus and DNA molecules
The transmission electron microscope (TEM) and scanning electron microscopes (SEM)
Vacuum chamber with chemically preserved specimens required to prevent....
electrons from scattering in the air
refocuses bent light, the image can be much clearer
Phase contrast microscopes
A stream of electrons is emitted instead of light with this microscope
TEM and SEM
cannot be used for live speciments
TEMs and SEMs (because of the vacuum chambers and chemicals)
made it easier to see same color organelles
fluorescent microscopy
requires thin slices of sample to provide a cross-section view
(TEM) Transmission Electron microscope
requires the process of staining certain structures in a cell with fluorescent proteins and shining a laser on them
Fluorescent microospy
Provdes 3D images by scanning their services
SEMs