ORDER OF DRAW, TUBES & ADDITIVES
TEST
LEGAL & ETHICAL CONSIDERATIONS
LABORATORY REQUIREMENTS & DEPARTMENTS
SAFETY & INFECTION CONTROL
TROUBLE SHOOTING & SPECIAL SITUATIONS
PREANALYTICAL ERRORS & ISSUES
100

What tube is a CBC collected in?

 Lavender Tube

100

What does FBS stand for? 

Fasting blood sugar

100

What agency is responsible for the health and safety of workers? 

OSHA

100

Where is the proper place for the tourniquet to be placed during a venipuncture? 

3 to 4 inches above the site

100

When should a phlebotomist wash their hands? 

Before and after the draw

100

What does warming the site do?

 Increases localized blood flow

100

What test do you draw without a tourniquet?

 Lactic acid

200

What tube Is an Erythrocyte Sedimentation Rate (ESR) drawn in? 

Lavender

200

What two analytes are included in an electrolyte panel? 

Sodium and potassium

200

Why is it important to ask for feedback when communicating with a patient? 

To make sure they understand what is being said

200

If the processing lab is backed up on resulting, what will you do with your samples?

Send them anyway

200

What is the recommended disinfectant to clean up blood, or other bodily fluid contamination?

Sodium Hypochlorite (bleach)

200

When drawing blood from a hemophiliac patient, instruct them to…

Hold pressure on the site for 2 minutes

200

Is it okay to use 70% isopropyl alcohol for cleansing the site when drawing blood for ethanol testing?

NO

300

Out of Red, green, light blue, and lavender top tubes which will produce serum? 

Red

300

What equipment is needed for a urine C&S? 

Sterile cup and alcohol wipe (towelette)

300

If a patient extends his or her arm, this is an example of what type of consent? 

Implied consent

300

When drawing multiple tubes, when would the tourniquet be released? 

As soon as blood flow is established

300

What PPE is required when drawing blood and handling blood products? 

Gloves

300

When drawing from a patient who has had a mastectomy where is the appropriate place to draw?

Opposite side in the hand

300

What can be caused by leaving the tourniquet on too long?

 Hemoconcentration

400

If asked to draw a red, light blue, blood cultures and lavender. Which is last?

Lavender

400

How do you collect a POC glucose test?

 Strip and a drop of blood

400

Informed consent is required for? 

Invasive surgery and Biopsy

400

How do you explain a phlebotomy procedure to a young child?

 In simple terms

400

What uses heat, steam, and chemicals? 

Sterilization

400

If a hematoma starts to form and the patient says it hurts, what should the Phlebotomist do? 

Stop the draw, apply pressure, reassess

400

What does the term hematoma mean? 

Blood leaking from the vessel under the skin

500

Red top is used for the following tests... 

Blood Pregnancy and Hepatic Panel

500

What test will you collect in an amber colored tube?

 Bilirubin

500

What agency mandates a written exposure plan and what contains documentation of chemical safety & precautions

OSHA & SDS log

500

What department is responsible for sterile supplies? 

Central Supply

500

What is the FIRST thing you will do in case of an accidental needle stick?

Wash the site for 30/60 seconds with soap and water 

500

If a patient is on blood thinners or an aspirin regimen what should be used after the blood draw?

 Coban wrap

500

What would be a reason for a light blue top to be rejected for processing?

QNS (quantity not sufficient)

600

Tiger top is used for a

Thyroid panel

600

What is the normal range for a fasting glucose for an adult?

 Anything under 100

600

If a coworker comes back late from lunch and asks you to cover for him or her, what will you do?

 Tell them you cannot cover for them.

600

What are the special handling requirements for an ammonia sample?  

Place on ice

600

When drawing from an HIV patient, what extra precautions do you need to take?

None, always wear gloves 

600

If you have an order to collect a Prothrombin Time (PT) specimen from a patient with IVs in both arms, where would you draw?

 The hand

600

If a specimen is lipemic, what can this be an indication of? 

A Patient not fasting

700

If a Dr. orders a CMP, CBC, PT and blood cultures what is the correct order of draw? Blood cultures, Lt blue, SST(Gold) and lavender 

 Blood cultures, Lt blue, SST(Gold) and lavender 

OR 

Blood Cultures PT, CMP, CBC

700

What is used to collect DNA samples by swabbing the inside of the cheek? 

Buccal Swab

700

What is it that states that a patient has the right to refuse medical procedures and be informed of all consequences in doing so? 

Patient bill of rights

700

What are the special handling requirements for a vitamin B12?

 Protect from light

700

What Bloodborne   Pathogen can remain on surfaces for up to 7 days if not properly disinfected? 

HBV (Hepatitis B)

700

What step is NO LONGER recommended for patients who pass out? 

Ammonia inhalants

700

If a patient presents to the lab for a fasting glucose blood draw but indicates he or she is NOT fasting, what should the Phlebotomist do?

 Draw anyway and document “non-fasting.”

800

What is the additive in a dark green tube? 

Sodium Heparin

800

When performing a bleeding timed test, how high should the blood pressure cuff be inflated to? 

40mm Hg

800

What agency mandates a written exposure plan? 

OSHA

800

At what age and weight will you no longer perform a heel puncture? 

Older than 12 months and more than 21 pounds

800

What is the #1 way to prevent cross contamination of micro-organisms from one patient to another? 

Hand washing (Hand decontamination)

800

If a patient faints during a blood draw, what is the vital sign that must be checked before you let them leave? 

Pulse

800

What is the normal BP for an adult? 

120/80

900

What is the substance at the bottom of a serum separator tube? 

Thixotropic gel

900

Which coagulation tests can be performed at bedside using a point of care instrument? 

PTT and INR

900

If a patient comes in asking for results, what should you do? 

Advise them to make an appt with their Dr.

900

Where does FDP (fibrin degradation products) go for processing? 

Coagulation Department

900

What type of isolation requires special handling and ventilation? 

Airborne Isolation

900

What is outside of the normal range for BP on an adult? 

139/94 (high)

1000

What color top would an aluminum be drawn in? 

Royal blue

1000

When instructing a patient to collect a stool sample, make sure that you instruct the patient to…

Do not mix stool and urine

1000

If you draw blood from a patient without his or her consent what can the Phlebotomist be charged with?

 Battery

1000

Once the blood draw is complete, what is the next step?

 Dispose of the needle into a puncture resistant container (sharps container)

1000

What is the most important part of blood culture collection? 

Aseptic procedure (cleansing of the site)

1000

What can happen if you draw an EDTA tube before a serum tube? 

Cross contamination resulting in high potassium levels, and false low calcium levels

1100

What color tubes are used to draw a Hemoglobin A1C, and a PT/PTT, and what is the correct order of draw? 

1. Light blue

2. Lavender

1100

How many tubes are needed for a 3-hour GTT? 

 4 tubes

1100

If you suspect child or elder abuse or neglect, what should you do?  

Notify your supervisor

1100

When do you change out the sharp’s container? 

3/4 full

1100

According to OSHA why do we activate the safety feature on a needle? 

To prevent accidental needle sticks

1100

Sweating, looking pale, and complaining of blurred vision can be signs of what? 

Vasovagal Syncope

1200

What is the proper blood to additive ratio for a light blue top tube?

9:1 (The blood to additive ratio is 9 parts blood to 1 part sodium citrate)

1200

How much urine would an adult female patient need to produce for STD testing? 

20/30 ml

1200

What is the purpose of patient ID numbers being on the tube labels? 

Avoiding confusing patients with the same first and last name

1200

What is the special handling required for paternity testing?  

Chain of custody

1200

What contains documentation of chemical safety and precautions?

 SDS log

1200

How long should a specimen drawn in an SST tube be allowed to sit and clot before centrifugation? 

30 to 60 minutes

1300

How long do you rotate a NP swab in each nostril when collecting a covid screening test?

 15 seconds

1300

What is the purpose of checking the arm band on an in-patient who has lab orders?

 Avoiding patient identification errors

1300

How often does a centrifuge need to be calibrated?

 Every 6 months

1300

How long can plasma sit at room temperature? 

8 Hours

1400

How long should a patient fast for a fasting blood sugar?

 8 hours

1400

What kind of checks need to be performed on POC equipment in a Dr.’s office? 

Quality Control Checks

1400

According to CLSI standards what is the maximum time a specimen (plasma or serum) can be left at room temp before centrifugation?  

2 hours

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