Tools of the Trade
What is the function of a micropipette?
Transfers small volumes of liquid.
What is a plasmid?
A circular DNA molecule used as a vector.
What is bacterial transformation?
The process of bacteria taking up foreign DNA.
What is the purpose of column chromatography?
To separate proteins based on properties.
What is the name of the wizarding school in Harry Potter?
Hogwarts
What material is commonly used for gel electrophoresis?
Agarose
What does the ori (origin of replication) do?
Allows the plasmid to replicate in bacterial cells.
What temperature is used for the heat shock method?
42°C
Why are multiple buffer solutions used in chromatography?
To selectively bind, wash, and elute the protein.
Who is the lead singer of the band Queen?
Freddie Mercury.
Which enzyme is used to cut DNA at specific sequences?
Restriction enzymes.
Why are antibiotic resistance genes included in plasmids?
To select for bacteria that have taken in the plasmid.
What happens if a bacterial cell does not take up the plasmid?
It will not grow on antibiotic plates.
What step releases the target protein in chromatography?
The elution step.
What is the capital of France?
Paris
What is the purpose of a DNA ladder in gel electrophoresis?
To compare fragment sizes.
What is recombinant DNA?
DNA that has been combined from different sources.
Why do transformed bacteria need to be grown on antibiotic plates?
To ensure only transformed bacteria survive.
How does size affect the speed of protein movement in chromatography?
Smaller proteins move faster.
In what year was the first iPhone released?
2007.
Why does DNA move toward the positive electrode during electrophoresis?
DNA is negatively charged and moves toward the positive electrode.
What enzyme seals DNA fragments together after restriction enzyme cutting?
DNA ligase.
What is the role of arabinose in gene expression?
It acts as an inducer to turn on gene expression.
Why is RFP (Red Fluorescent Protein) useful in biotechnology?
It allows easy visualization of successful expression.
What is the highest-grossing movie of all time (as of 2023)
Avatar (2009).