LOD & LOQ,
Spiked Recovery
Spiked Recovery
If you are trying to determined if there is a real signal, you calculate this.
What is LOD or Limit of Detection?
When dealing with an external calibration curve, do you solve for the x-component relative to y = signal or y = 0?
What is y = signal?
I am conducting an experiment to see which cookie brand has the most cookies per package. I am looking at Chips Ahoy, Oreo, and Nutter Butter, which test should I use?
What is ANOVA?
If my standard deviations are determined the same, what test should I use as a follow-up to check if the means are the same?
What is the standard T-test?
A lower standard deviation will result in a more ______ Gaussian curve.
What is narrower?
To determine the minimum signal do you use the k of the LOD (3) or of the LOQ (10)?
What is the LOD (3)?
"Components can enhance or suppress analyte signal" is the definition of what phenomenon?
What is the Matrix Effect?
A scientist is trying to compare the precision of two fluorimeters, which statistical test should she perform?
What is an F-test?
I am conducting an experiment where I measure blood glucose levels before eating and after eating a cookie. What statistical test should I perform?
What is a paired t-test?
What does the variance look like mathematically?
What is s2?
What are the three value you need to determine the LOQ?
What are: k (10), standard deviation (sbl), and the sensitivity (m)?
What is 4.05?
If the standard deviations are determined to be different, should we proceed using a pooled standard deviation for the test to determine if the means are different?
What is No?
If working I am working in a lab, and I need to know how many flies to prepare use in my experiment, I would use this test.
What is Power Analysis?
If we were to go from a 99% confidence to a 95% confidence, what would happen to our confidence range?
What is "it will get smaller"?
If you have a spiked recovery greater than 100% the most likely source of the additional percent is this.
What is contamination?
Look at the image, what kind of calibration curve is this?
External Calibration Curve
I have the following data set:
0.04, 0.01, 0.06, 0.03, .05, 0.04, 0.05, 0.05, 0.04, 0.03, 0.05, and 0.06
There is a standard deviation of 0.015 and a mean of 0.0425, is there an outlier and what should we do with the data point?
What is no and keep it?
I want to measure the mean striation level in zebrafish has been significantly increased to the 95% confidence level, which test should I use?
What is a one tailed t-test?
Standard deviation, SEM, and confidence intervals can all be used to gauge the what of an experiment?
What is precision?
A scientist is testing a water sample for the amount of lead present. The original sample is measured to contain 21.5 µg/L of lead. The scientist then adds a known amount of 10.0 µg/L of lead to another portion of the same sample. After testing the spiked sample, the measured concentration is 28.2 µg/L. What is the percent spiked recovery?
What is 67.0%
A sample was subject to a 5x dilution. The diluted sample was then used to generate a standard addition calibration curve. The curve used the following equation, where y is in concentration (uM).
y = 2.97x + 5.73
What was the initial concentration of analyte in the undiluted sample?
What is 9.65?
If a mean is 34 and the standard deviation is 1.62, what is the percent probability that my measurement is above 1.2 or below -1.8 standard deviations away from the mean?
What is 15.1%?
I learned the standard deviations were the same, now I am checking for mean. I am comparing two datasets, the first had 13 samples with a mean of 29.4 and std of 1.2, the second had 11 samples with a mean of 32.1 and std of 0.9. Are the means the same to the 95% confidence level, and what does this mean for the null hypothesis?
What is "no, the means are different" and we reject the null hypothesis?
What is the standard deviation of a dataset, if the mean is 11.2, the sample size is 30, and the standard error of the mean is 0.6?
What is 3.29?