Chem Synthesis & NMR
This describes the formation of a compound that contains an alkyl group.
What is "esterification"?
These are the two main purification strategies when using solid-phase extraction.
What are bind-wash-elute & passthrough cleanup?
Internal standard can be used to quantify fatty acid methyl esters because every FAME exhibits the same ______(THIS)_____. This is then eliminated from the equation used for calculating amounts.
Response factor
This is a form of ELISA wherein antibody is bound to a substrate, sample is added, and more antibodies are used for detection.
What is sandwich ELISA?
This was a bonus last year...
This analytical test is most useful for seeing whether there was any starting material left at the end of the reaction.
What is the ferric chloride assay?
What is Empower?
Fats cannot be directly injected into gas chromatography systems without methylation because they are not __(THIS)____.
What is volatile?
This step of the ELISA prevents antibody from binding to off-target sites.
What is blocking?
These were the two media types used in the mixed-mode SPE cartridges employed during anthocyanin purification.
What are cation exchange and C18 resins?
This must be applied in order for an energy transition to exist between spin states in a nucleus.
What is an applied magnetic field.
BONUS- This is Marla's dog's name
Salty (Salt, Saltydog, Dude)
These two steps are required for Quechers preparation of pesticides.
What are extraction (using salts) and purification (using dSPE)?
This rarely-used form of ELISA has the advantage of being excellent for detecting very small antigens
What is competitive ELISA?
Calculation of total anthocyanin concentration in samples using Beer Lambert equation required these three values?
What are absorbance, pathlength, and molar absorptivity coefficient.
The chemical shift, how far to the left of the zero, of an NMR signal is caused by this.
What is the degree of shielding of the hydrogen.
In reverse phase chromatography, this is the most polar phase.
What is the mobile phase?
This is the gradient that varies during GC analysis in order to elute compounds from the column.
What is oven temperature?
When performing ELISA, this step should be taken if it is anticipated that there will be a very high amount of unknown antigen in the samples.
What is sample dilution?
This measure of process efficiency is represented by the total amount of analyte entering a system compared to the total recovery.
What is mass balance? (yield)
These are responsible for the splitting of the signal during Hydrogen NMR.
To change the resolution of peaks on UPLC, these two method parameters could be considered.
What are gradient and flow rate?
This GC parameter only applies a portion of a sample into the GC column, which helps in reducing the size of the solvent peak.
What is split injection?
This property of TNFa made it more suitable for quantification by ELISA than by Western blot.
What is being a "released factor"?
These are the central aglycone structures of anthocyanin molecules.
What are anthocyanidins?