Chem Synthesis & NMR
This term describes the reaction between an alcohol and a carboxylic acid to form a new functional group.
What is "esterification"?
These are the two main purification strategies used in solid-phase extraction.
What are bind-wash-elute & passthrough cleanup?
n internal standard can be used to quantify fatty acid methyl esters because all FAMEs share this characteristic, which therefore cancels out in calculations.
What is response factor
This type of ELISA uses a capture antibody bound to a solid phase, followed by sample addition and a detection antibody.
What is sandwich ELISA?
This acid is added during anthocyanin extraction to stabilize the cation form of the pigments.
What is formic acid?
This technique is commonly used to assess reaction completeness by comparing reactant and product mobility on a plate.
What is TLC?
BONUS:
This catchphrase is guaranteed to be said by Jessy at some point every day.
What is "what do you think"?
Fats cannot be directly injected into a gas chromatograph because they lack this property.
What is volatility?
This step in ELISA prevents antibodies from binding to non-specific sites on the plate.
What is blocking?
These two media types were used in the mixed-mode SPE cartridges during anthocyanin purification.
What are cation exchange and C18 resins?
This must be applied to a nucleus in order for energy transitions between spin states to occur in NMR spectroscopy.
What is an external magnetic field.
This UPLC parameter determines how strongly analytes interact with the stationary phase by altering mobile-phase composition over time.
What is gradient elution?
These two steps are required in QuEChERS preparation for pesticide analysis.
What are extraction (with salts) and purification (with dSPE)?
This less common form of ELISA is particularly effective for detecting very small antigens.
What is competitive ELISA?
These three values are required to calculate total anthocyanin concentration using the Beer–Lambert law.
What are absorbance, pathlength, and molar absorptivity coefficient.
The chemical shift of a proton signal depends on this factor, which reflects how the nucleus is hidden by surrounding electrons.
What is the degree of hydrogen shielding?
In reverse-phase chromatography, this phase is the more polar of the two.
What is the mobile phase?
This parameter is increased during a GC run to promote elution of higher-boiling compounds.
What is oven temperature?
This step should be taken when very high levels of antigen are expected in the sample.
What is sample dilution?
This term represents the efficiency of a process by comparing total analyte recovered to total analyte introduced.
What is mass balance? (yield)
These neighboring atoms cause splitting of a hydrogen signal in NMR.
What are adjacent hydrogens?
Adjusting these two method parameters can improve the resolution of peaks in UPLC analysis.
What are gradient and flow rate?
This injection mode introduces only part of the sample into the GC column, reducing solvent overloading.
What is split injection?
This characteristic of TNF-α made it more suitable for ELISA quantification than for Western blotting.
What is being a secreted/released factor?
These structures form the aglycone backbone of anthocyanin molecules.
What are anthocyanidins?