Molarity(M)
The unit used to express Molarity in solutions prepared for cell culture media. (HINT* What units do M=?)
What is:
moles per liters (mol/L)
This number is known as Avogadro’s number
What is:
6.02 × 10²³
What does the unit volume per volume percent (v/v%) describe?
This unit describes liquid-to-liquid solutions
Serial dilutions allow for a range of decreasing...
What is:
Concentrations
How many students are in Biotech Class of 2027?
52
A buffer with 0.25 mol of Tris in 0.5L of solution has what molarity(M)?
What is:
0.5M
The number of moles in 12 grams of carbon-12
What is:
1 mol
A solution is made with 10 mL ethanol and 90 mL water.
What is:
10% (v/v)
Each step in a serial dilution reduces concentration by consistent factor known as
What is:
Dilution factor
What did Ms. Brooks get her Master of Science degree in?
Biotechnology
You dissolve 5 moles of a buffer component into 2.5 liters of solution. What is the molarity?
What is:
2 M
Moles are used in biotechnology because they allow scientists to compare substances based on the...
What is:
number of particles
A student dissolves 10 g of agarose in 200 mL buffer.
What is:
5% (w/v)
A 1:10 serial dilution is performed three times. What is the final dilution? AND dilution factor?
What is:
final dilution is 1:1000, dilution factor is 1000
What is the Academy of Biotechnology's ranking with NAF?
Distinguished
To make 1 liter of 0.1 M PBS, this amount of total solute (in moles) is required.
What isL
0.1 moles
A buffer requires 0.25 moles of Tris. Tris has a molar mass of 121 g/mol. How many grams are required?
What is:
30.25 g
To prepare 1 liter of a 2% (w/v) glucose solution, this mass of glucose is needed.
What is:
20 g
A bacterial culture is diluted 1:10, then 1:10 again. What is the total dilution factor?
What is:
1:100
The Director of the Academy of Biotechnology, Dr. Adjei, has a PhD in?
Genetics
A 2 M stock solution is diluted to make 1 liter of a 0.5 M working solution for cell culture. How much of the stock solution is needed? (*HINT = need mL)
What is:
250mL
A researcher doubles the mass of a compound added to a reaction but keeps the number of moles constant. How is this possible?
What is:
The molar mass must be different
w/v% is often used in biotechnology because it avoids this type of calculation.
What is:
Molar mass calculations
Serial dilutions are critical in Biotech for this purpose
What is:
counting bacterial colonies or estimating concentration
BACE stands for
Biotechnology Aptitude and Competency Exam